Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Metabacillus fastidiosus |
General Stability | Organism |
---|---|
after modification with monomethoxy-poly(ethylene glycol)-5000, the recombinant intracellular uricase shows residual activity of about 65% | Metabacillus fastidiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenine | negligible inhibition at 0.5 mM at pH 9.2 | Metabacillus fastidiosus | |
Cu2+ | strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM | Metabacillus fastidiosus | |
Fe2+ | strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM | Metabacillus fastidiosus | |
hypoxanthine | negligible inhibition at 0.5 mM at pH 9.2 | Metabacillus fastidiosus | |
oxonate | - |
Metabacillus fastidiosus | |
xanthine | - |
Metabacillus fastidiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
Urate | unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 | Metabacillus fastidiosus | |
0.8 | - |
Urate | unmodified enzyme, 50 mM borate buffer, 40°C, pH 9.2 | Metabacillus fastidiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strong activation at 10 mM | Metabacillus fastidiosus | |
K+ | slight activation at 10 mM | Metabacillus fastidiosus | |
Mg2+ | strong activation at 10 mM | Metabacillus fastidiosus | |
Na+ | slight activation at 10 mM | Metabacillus fastidiosus | |
NH4Cl | slight activation at 10 mM | Metabacillus fastidiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35780 | - |
MALDI-TOF mass spectrometry | Metabacillus fastidiosus |
35790 | - |
calculated from amino acid sequence | Metabacillus fastidiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metabacillus fastidiosus | C5HDG5 | - |
- |
Metabacillus fastidiosus ATCC 29604 | C5HDG5 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography | Metabacillus fastidiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Metabacillus fastidiosus | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Metabacillus fastidiosus ATCC 29604 | 5-hydroxyisourate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
uricase | - |
Metabacillus fastidiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at 40°C in sodium borate buffer at pH 9.2, the unmodified uricase shows a thermo-inactivation half-life of about 40 h. Modification of the uricase by monomethoxy-poly(ethylene glycol)-350 slightly enhances its thermostability, and modification by monomethoxy-poly(ethylene glycol)-5000 increases its thermo-inactivation half-life to over 85 h at 40°C in sodium borate buffer at pH 9.2 | Metabacillus fastidiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
the unmodified uricase has a pH optimum of slightly below pH 9.2 which is not altered by modification with NHS esters of monomethoxy-poly(ethylene glycol)-5000 or monomethoxy-poly(ethylene glycol)-350 | Metabacillus fastidiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
oxonate | unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 | Metabacillus fastidiosus | |
0.055 | - |
xanthine | unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 | Metabacillus fastidiosus | |
0.1 | - |
xanthine | unmodified enzyme, 50 mM borate buffer, 40°C, pH 9.2 | Metabacillus fastidiosus |