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Literature summary for 1.7.3.3 extracted from

  • Zhang, C.; Yang, X.; Feng, J.; Yuan, Y.; Li, X.; Bu, Y.; Xie, Y.; Yuan, H.; Liao, F.
    Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus (2010), Biosci. Biotechnol. Biochem., 74, 1298-1301.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Metabacillus fastidiosus

General Stability

General Stability Organism
after modification with monomethoxy-poly(ethylene glycol)-5000, the recombinant intracellular uricase shows residual activity of about 65% Metabacillus fastidiosus

Inhibitors

Inhibitors Comment Organism Structure
adenine negligible inhibition at 0.5 mM at pH 9.2 Metabacillus fastidiosus
Cu2+ strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM Metabacillus fastidiosus
Fe2+ strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM Metabacillus fastidiosus
hypoxanthine negligible inhibition at 0.5 mM at pH 9.2 Metabacillus fastidiosus
oxonate
-
Metabacillus fastidiosus
xanthine
-
Metabacillus fastidiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
Urate unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 Metabacillus fastidiosus
0.8
-
Urate unmodified enzyme, 50 mM borate buffer, 40°C, pH 9.2 Metabacillus fastidiosus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strong activation at 10 mM Metabacillus fastidiosus
K+ slight activation at 10 mM Metabacillus fastidiosus
Mg2+ strong activation at 10 mM Metabacillus fastidiosus
Na+ slight activation at 10 mM Metabacillus fastidiosus
NH4Cl slight activation at 10 mM Metabacillus fastidiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35780
-
MALDI-TOF mass spectrometry Metabacillus fastidiosus
35790
-
calculated from amino acid sequence Metabacillus fastidiosus

Organism

Organism UniProt Comment Textmining
Metabacillus fastidiosus C5HDG5
-
-
Metabacillus fastidiosus ATCC 29604 C5HDG5
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography Metabacillus fastidiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Metabacillus fastidiosus 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Metabacillus fastidiosus ATCC 29604 5-hydroxyisourate + H2O2
-
?

Synonyms

Synonyms Comment Organism
uricase
-
Metabacillus fastidiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
at 40°C in sodium borate buffer at pH 9.2, the unmodified uricase shows a thermo-inactivation half-life of about 40 h. Modification of the uricase by monomethoxy-poly(ethylene glycol)-350 slightly enhances its thermostability, and modification by monomethoxy-poly(ethylene glycol)-5000 increases its thermo-inactivation half-life to over 85 h at 40°C in sodium borate buffer at pH 9.2 Metabacillus fastidiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
the unmodified uricase has a pH optimum of slightly below pH 9.2 which is not altered by modification with NHS esters of monomethoxy-poly(ethylene glycol)-5000 or monomethoxy-poly(ethylene glycol)-350 Metabacillus fastidiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
oxonate unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 Metabacillus fastidiosus
0.055
-
xanthine unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 Metabacillus fastidiosus
0.1
-
xanthine unmodified enzyme, 50 mM borate buffer, 40°C, pH 9.2 Metabacillus fastidiosus