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Literature summary for 1.7.3.3 extracted from

  • Colloc'h, N.; Gabison, L.; Monard, G.; Altarsha, M.; Chiadmi, M.; Marassio, G.; Sopkova-de Oliveira Santos, J.; El Hajji, M.; Castro, B.; Abraini, J.H.; Prange, T.
    Oxygen pressurized X-ray crystallography: probing the dioxygen binding site in cofactorless urate oxidase and implications for its catalytic mechanism (2008), Biophys. J., 95, 2415-2422.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Aspergillus flavus

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme in complex with inhibitor 8-azaxanthine in presence of O2 or Cl-, batch technique at room temperature, 10-15 mg/ml protein with an excess of 0.5-2 mg/ml of 8-azaxanthin in 50 mM Tris/HCl, pH 8.5, in the presence of 5-8% w/v PEG 8000 and 0.05 M NaCl, 24-48 h, X-ray diffraction structure determination and analysis at 1.6-1.7 A resolution Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
8-Azaxanthine competitive inhibitor, binding structure, overview Aspergillus flavus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the catalytic mechanism of UOX does not imply any metal ion Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Aspergillus flavus
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus flavus Q00511
-
-

Reaction

Reaction Comment Organism Reaction ID
urate + O2 + H2O = 5-hydroxyisourate + H2O2 catalytic mechanism, overview Aspergillus flavus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O dioxygen-binding site structure, overview Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
Uox
-
 Aspergillus flavus

Cofactor

Cofactor Comment Organism Structure
additional information the catalytic mechanism of UOX does not imply any cofactor Aspergillus flavus