Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, Escherichia coli harboring pUOD1 produces 20fold higher uricase than the original Arthrobacter strain, even without an inducer | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | enzyme contains copper, inhibited by excessive addition of Cu2+, 98% inhibition by 1 mM CuCl2, 97% inhibition by 1 mM CuSO4 | Arthrobacter globiformis | |
additional information | no inhibition by 1 mM Fe(ClO4)3 or 1 mM AgNO3 | Arthrobacter globiformis | |
ZnCl2 | 1 mM, 91% inhibition | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
Urate | pH 7.0, 37°C | Arthrobacter globiformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Arthrobacter globiformis | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | enzyme contains copper, inhibited by excessive addition of Cu2+ | Arthrobacter globiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Arthrobacter globiformis |
33858 | - |
4 * 33858, calculation from nucleotide sequence | Arthrobacter globiformis |
117000 | - |
gel filtration | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Arthrobacter globiformis | enzyme production is induced by addition of uric acid to the culture medium | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | Arthrobacter globiformis FERM BP-360 | enzyme production is induced by addition of uric acid to the culture medium | 5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
FERM BP-360 | - |
Arthrobacter globiformis FERM BP-360 | - |
FERM BP-360 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | enzyme production is induced by addition of uric acid to the culture medium | Arthrobacter globiformis | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | enzyme production is induced by addition of uric acid to the culture medium | Arthrobacter globiformis FERM BP-360 | 5-hydroxyisourate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Arthrobacter globiformis |
tetramer | 4 * 33858, calculation from nucleotide sequence | Arthrobacter globiformis |
Synonyms | Comment | Organism |
---|---|---|
Urate oxidase | - |
Arthrobacter globiformis |
uricase | - |
Arthrobacter globiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Arthrobacter globiformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | 11 | stable | Arthrobacter globiformis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arthrobacter globiformis | isoelectric focusing, pH gradient 3.5-9.5 | - |
4.96 |