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Literature summary for 1.7.3.3 extracted from
- Kinetic mechanism and cofactor content of soybean root nodule urate oxidase (1997), Biochemistry, 36, 4731-4738.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 9-methyluric acid | competitive versus urate | Glycine max |  |
| xanthine | competitive versus urate, noncompetitive versus O2 | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0098 | - |
uric acid | - |
Glycine max | |
| 0.061 | - |
O2 | - |
Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35050 | - |
deduced from nucleotide sequence of cDNA | Glycine max |
| 35060 | - |
MALDI-TOF mass spectrometry | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Glycine max | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root nodule | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
- |
Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, several weeks | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Glycine max | 5-hydroxyisourate + H2O2 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactors | Glycine max |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 1-methylurate, 3-methylurate, 7-methylurate do not inhibit significantly | Glycine max | |
| 4.5 | - |
xanthine | competitive with urate | Glycine max | |
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