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Literature summary for 1.7.3.1 extracted from

  • Valley, M.P.; Tichy, S.E.; Fitzpatrick, P.F.
    Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase (2005), J. Am. Chem. Soc., 127, 2062-2066.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.3.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
cyanide inactivation of the enzyme by trapping of the electrophilic imine reaction intermediate, formation of a cyanoalkyl intermediate, reaction with the substrate after proton abstraction but before flavin oxidation Fusarium oxysporum
Sodium cyanide 2 mM and above, complete inhibition Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic competency of the cationic imine enzyme intermediate Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fusarium oxysporum

Reaction

Reaction Comment Organism Reaction ID
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O mechanism, involves removal of a proton from the nitroalkane, forming a carbanion which adds to the N5 of the flavin, elimination of nitrite from the resulting adduct forms an electrophilic imine intermediate which can be attacked by hydroxide Fusarium oxysporum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitrobutane + O2 + H2O
-
 Fusarium oxysporum butyraldehyde + nitrite + H2O2
-
 ?
1-nitrohexane + O2 + H2O
-
 Fusarium oxysporum hexanal + nitrite + H2O2
-
 ?
additional information analysis of reaction intermediates formed by mass spectrometry and gel filtration Fusarium oxysporum ?
-
 ?
nitroethane + H2O + O2
-
 Fusarium oxysporum acetaldehyde + nitrite + H2O2
-
 ?
nitroethane + O2 + H2O
-
 Fusarium oxysporum ethanal + nitrite + H2O2
-
 ?
phenylnitromethane + O2 + H2O
-
 Fusarium oxysporum ? + nitrite + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
NAO
-
 Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Fusarium oxysporum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Fusarium oxysporum
FAD formation of 5-nitroalkyl-FAD intermediates, structure in cyanide inactivated enzyme Fusarium oxysporum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inactivation kinetics with different substrates Fusarium oxysporum