Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme induction by growth on nitroethane | Fusarium oxysporum |
Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant D402E enzymes | Fusarium oxysporum |
Protein Variants | Comment | Organism |
---|---|---|
D402E | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, mutation has no effect on the rate of reaction of the reduced enzyme with oxygen, reaction mechanism analysis | Fusarium oxysporum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and rapid reaction kinetics of wild-type and mutant enzymes | Fusarium oxysporum | |
0.03 | - |
1-nitrobutane | recombinant wild-type enzyme, pH 8.0, 30°C | Fusarium oxysporum | |
0.18 | - |
1-nitrobutane | recombinant mutant D403E, pH 8.0, 30°C | Fusarium oxysporum | |
2.3 | - |
nitroethane | recombinant wild-type enzyme, pH 8.0, 30°C | Fusarium oxysporum | |
14.6 | - |
nitroethane | recombinant mutant D403E, pH 8.0, 30°C | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O | Asp402 acts as the active site base of the enzyme, mechanism, substrate CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-nitrobutane + O2 + H2O | - |
Fusarium oxysporum | butyraldehyde + nitrite + H2O2 | - |
? | |
additional information | enzyme catalyzes the oxidation of primary and secondary nitroalkanes to the respective aldehydes or ketones, releasing nitrite | Fusarium oxysporum | ? | - |
? | |
nitroethane + O2 + H2O | O2 is delivered from air-saturated buffer in the assay reaction | Fusarium oxysporum | ethanal + nitrite + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the acyl-CoA dehydrogenase family of enzymes | Fusarium oxysporum |
NAO | - |
Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Fusarium oxysporum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Fusarium oxysporum |