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Literature summary for 1.7.3.1 extracted from

  • Valley, M.P.; Fitzpatrick, P.F.
    Reductive half-reaction of nitroalkane oxidase: effect of mutation of the active site apartate to glutamate (2003), Biochemistry, 42, 5850-5856.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme induction by growth on nitroethane Fusarium oxysporum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant D402E enzymes Fusarium oxysporum

Protein Variants

Protein Variants Comment Organism
D402E site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, mutation has no effect on the rate of reaction of the reduced enzyme with oxygen, reaction mechanism analysis Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
additional information no substrate inhibition Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state and rapid reaction kinetics of wild-type and mutant enzymes Fusarium oxysporum
0.03
-
1-nitrobutane recombinant wild-type enzyme, pH 8.0, 30°C Fusarium oxysporum
0.18
-
1-nitrobutane recombinant mutant D403E, pH 8.0, 30°C Fusarium oxysporum
2.3
-
nitroethane recombinant wild-type enzyme, pH 8.0, 30°C Fusarium oxysporum
14.6
-
nitroethane recombinant mutant D403E, pH 8.0, 30°C Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O Asp402 acts as the active site base of the enzyme, mechanism, substrate CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitrobutane + O2 + H2O
-
Fusarium oxysporum butyraldehyde + nitrite + H2O2
-
?
additional information enzyme catalyzes the oxidation of primary and secondary nitroalkanes to the respective aldehydes or ketones, releasing nitrite Fusarium oxysporum ?
-
?
nitroethane + O2 + H2O O2 is delivered from air-saturated buffer in the assay reaction Fusarium oxysporum ethanal + nitrite + H2O2
-
?

Synonyms

Synonyms Comment Organism
More enzyme belongs to the acyl-CoA dehydrogenase family of enzymes Fusarium oxysporum
NAO
-
Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Fusarium oxysporum