Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | addition of 0.035 mM, 0.085 mM, or 0.35 mM cyanide results in 25%, 50%, and 95% inhibition, respectively | Candidatus Brocadia anammoxidans | |
H2O2 | 0.0004 mM, 0.004 mM, and 0.04 mM results in a loss of activity of 30%, 75%, and 90%, respectively | Candidatus Brocadia anammoxidans | |
hydrazine | competes strongly with hydroxylamine. Addition of 0.015 mM, 0.035 mM, and 0.35 mM results in 15%, 50%, and 85% inhibition, respectively | Candidatus Brocadia anammoxidans | |
additional information | sodium azide, methanol, sodium nitrite, and the chelating agent EDTA do not inhibit enzyme activity | Candidatus Brocadia anammoxidans | |
SDS | activity in the presence of 0.75%, 3%, and 8% SDS is reduced by 20%, 50%, and 95%, respectively | Candidatus Brocadia anammoxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
hydrazine | pH 8.0, 35°C, reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate | Candidatus Brocadia anammoxidans | |
0.026 | - |
hydroxylamine | pH 8.0, 35°C, reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate | Candidatus Brocadia anammoxidans | |
0.127 | - |
hydroxylamine | pH 8.0, 65°C, reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate | Candidatus Brocadia anammoxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Candidatus Brocadia anammoxidans |
150000 | - |
gel filtration | Candidatus Brocadia anammoxidans |
183000 | - |
nondenaturing PAGE | Candidatus Brocadia anammoxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Candidatus Brocadia anammoxidans | the enzyme is involved in the pathway of anaerobic ammonium oxidation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candidatus Brocadia anammoxidans | - |
hydroxylamine oxidoreductase is purified and characterized from an anaerobic ammonium-oxidizing (Anammox) enrichment culture | - |
Purification (Comment) | Organism |
---|---|
- |
Candidatus Brocadia anammoxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrazine + 2,6-dichlorophenolindophenol | - |
Candidatus Brocadia anammoxidans | N2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
hydrazine + ferricyanide | ferricyanide as the electron acceptor results in the highest specific activity. Hydrazine is solely converted to N2 | Candidatus Brocadia anammoxidans | N2 + ferrocyanide | - |
? | |
hydrazine + ferricytochrome | horse heart cytochrome. Hydrazine is solely converted to N2 | Candidatus Brocadia anammoxidans | N2 + ferrocytochrome | - |
? | |
hydrazine + methylthiazolyltetrazolium bromide | reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate. Hydrazine is solely converted to N2 | Candidatus Brocadia anammoxidans | N2 + reduced methylthiazolyltetrazolium bromide | - |
? | |
hydroxylamine + 2,6-dichlorophenolindophenol | horse heart cytochrome. The conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected | Candidatus Brocadia anammoxidans | N2O + reduced 2,6-dichlorophenolindophenol | small amounts of NO are also formed | ? | |
hydroxylamine + ferricyanide | ferricyanide as the electron acceptor results in the highest specific activity. The conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected | Candidatus Brocadia anammoxidans | N2O + ferrocyanide | small amounts of NO are also formed | ? | |
hydroxylamine + ferricytochrome | horse heart cytochrome. The conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected | Candidatus Brocadia anammoxidans | N2O + ferrocytochrome | small amounts of NO are also formed | ? | |
hydroxylamine + methylthiazolyltetrazolium bromide | the conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected | Candidatus Brocadia anammoxidans | N2O + reduced methylthiazolyltetrazolium bromide | small amounts of NO are also formed | ? | |
additional information | the enzyme is involved in the pathway of anaerobic ammonium oxidation | Candidatus Brocadia anammoxidans | ? | - |
? | |
additional information | methanol is not a substrate, no activity with NAD+, benzyl viologen and Wurster's blue. The enzyme from anaerobic ammonium-oxidizing (Anammox) enrichment culture is also capable of reducing NO to N2O with a specific activity of 2.9 units/mg at room temperature. Reduction of nitrite in the presence of reduced methyl viologen results in the production of N2O and some NO (below 5%). No other nitrogen compounds are detected. In the presence of ascorbate and PMS, no nitrite conversion is observed | Candidatus Brocadia anammoxidans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Candidatus Brocadia anammoxidans |
Synonyms | Comment | Organism |
---|---|---|
HAO | - |
Candidatus Brocadia anammoxidans |
hydroxylamine oxidoreductase | - |
Candidatus Brocadia anammoxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Candidatus Brocadia anammoxidans |
65 | - |
highest activity with hydroxylamine as substrate | Candidatus Brocadia anammoxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Candidatus Brocadia anammoxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 1 mol of enzyme contains 26 mol hemes | Candidatus Brocadia anammoxidans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candidatus Brocadia anammoxidans | isoelectric focusing | - |
5.5 |