KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
ferricyanide | pH 7.8, temperature not specified in the publication | Nitrosococcus oceani | |
0.0011 | - |
ferricytochrome c-554 | source of substrate Nitrosococcus oceani, pH 7.8, temperature not specified in the publication | Nitrosococcus oceani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
x * 180000, SDS-PAGE | Nitrosococcus oceani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrosococcus oceani | M5DCM0 | - |
- |
Nitrosococcus oceani NS58 | M5DCM0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Nitrosococcus oceani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + ferricytochrome c-554 + H2O | - |
Nitrosococcus oceani | nitrite + ferrocytochrome c-554 + H+ | - |
? | |
hydroxylamine + ferricytochrome c-554 + H2O | - |
Nitrosococcus oceani NS58 | nitrite + ferrocytochrome c-554 + H+ | - |
? | |
hydroxylamine + H2O + 2 ferricyanide | - |
Nitrosococcus oceani | nitrite + 2 ferrocyanide + 5 H+ | - |
? | |
hydroxylamine + H2O + 2 ferricyanide | - |
Nitrosococcus oceani NS58 | nitrite + 2 ferrocyanide + 5 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 180000, SDS-PAGE | Nitrosococcus oceani |
More | the enzyme is composed of a homotrimer of subunits with molecular weight of 62000 Da covalently bound to each other. The bonds are mediated by prosthetic heme c molecules | Nitrosococcus oceani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
ferricytochrome c-554 | source of substrate Nitrosococcus oceani, pH 7.8, temperature not specified in the publication | Nitrosococcus oceani | |
111 | - |
ferricyanide | pH 7.8, temperature not specified in the publication | Nitrosococcus oceani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | purified enzyme shows typical cytochrome-like absorption spectra, but with a peculiar peak at 468 nm in the reduced state that is due to a P460 moiety corresponding to a modified heme c molecule | Nitrosococcus oceani | |
heme | protein contains at least 16 hemes c per molecule | Nitrosococcus oceani |