Literature summary for 1.7.2.4 extracted from

Prudencio, M.; Pereira, A.S.; Tavares, P.; Besson, S.; Cabrito, I.; Brown, K.; Samyn, B.; Devreese, B.; Van Beeumen, J.; Rusnak, F.; Fauque, G.; Moura, J.J.G.; Tegoni, M.; Cambillau, C.; Moura, I.
Purification, Characterization, and Preliminary Crystallographic Study of Copper-Containing Nitrous Oxide Reductase from Pseudomonas nautica 617 (2000), Biochemistry, 39, 3899-3907.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
2.4 A resolution, preliminary study	Marinobacter nauticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu	six copper atoms per monomer arranged in two centers named CuA and CuZ, 10.7 atoms per dimer	Marinobacter nauticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	2 * 65000, SDS-PAGE	Marinobacter nauticus
65373	-	2 * 65373, ESI mass spectrometry	Marinobacter nauticus
120000	-	gel filtration	Marinobacter nauticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nitrous oxide + reduced acceptor	Marinobacter nauticus	-	nitrogen + H2O + acceptor	-	?
nitrous oxide + reduced acceptor	Marinobacter nauticus 617	-	nitrogen + H2O + acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Marinobacter nauticus	-	-	-
Marinobacter nauticus 617	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
two forms a purple and a blue form	Marinobacter nauticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
23	-	blue-coloured form	Marinobacter nauticus
55	-	purple coloured form	Marinobacter nauticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrous oxide + reduced acceptor	-	Marinobacter nauticus	nitrogen + H2O + acceptor	-	?
nitrous oxide + reduced acceptor	-	Marinobacter nauticus 617	nitrogen + H2O + acceptor	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 65000, SDS-PAGE	Marinobacter nauticus
homodimer	2 * 65373, ESI mass spectrometry	Marinobacter nauticus

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Release 2023.1 (January 2023)