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Literature summary for 1.7.2.3 extracted from

  • Iobbi-Nivol, C.; Pommier, J.; Simala-Grant, J.; Mejean, V.; Giordano, G.
    High substrate specificity and induction characteristics of trimethylamine N-oxide reductase of Escherichia coli (1996), Biochim. Biophys. Acta, 1294, 77-82.
    View publication on PubMed
Search for more literature for 1.7.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 4-methylmorpholine N-oxide
-
 Escherichia coli
0.07
-
 Trimethylamine N-oxide
-
 Escherichia coli
0.26
-
 alpha-picoline N-oxide
-
 Escherichia coli
0.32
-
 gamma-picoline N-oxide
-
 Escherichia coli
0.75
-
 3-hydroxypyridine N-oxide
-
 Escherichia coli
1.14
-
 N,N-dimethylhexylamine N-oxide
-
 Escherichia coli
1.25
-
 N,N-dimethyldodecylamine N-oxide
-
 Escherichia coli
1.86
-
 Pyridine N-oxide
-
 Escherichia coli
2.01
-
 2-chloropyridine N-oxide
-
 Escherichia coli
2.15
-
 4-chloropyridine N-oxide
-
 Escherichia coli
4.74
-
 N-cyclohexyldimethylamine N-oxide
-
 Escherichia coli
5.43
-
 3-picoline N-oxide
-
 Escherichia coli
7.39
-
 hydroxylamine N-oxide
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 inducible enzyme
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM and a concentration 20 mM of 2-hydroxypyridine N-oxide Escherichia coli
34
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM Escherichia coli
35
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM and a concentration 20 mM of tetramethylenesulfoxide Escherichia coli
36
-
 purified enzyme, assay performed in 100 mM phosphate buffer, pH 6.8 with a trimethylamine N-oxide concentration 0.5 mM and a concentration 20 mM of picolinic acid N-oxide or a concentration 20 mM of dimethylsulfoxide Escherichia coli
105
-
 purified enzyme, with trimethylamine N-oxide as substrate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloropyridine N-oxide + electron donor
-
 Escherichia coli 2-chloropyridine + oxidized electron donor + H2O
-
 ?
3-hydroxypyridine N-oxide + electron donor
-
 Escherichia coli 3-hydroxypyridine + oxidized electron donor + H2O
-
 ?
3-picoline N-oxide + electron donor
-
 Escherichia coli 3-picoline + oxidized electron donor + H2O
-
 ?
4-chloropyridine N-oxide + electron donor
-
 Escherichia coli 4-chloropyridine + oxidized electron donor + H2O
-
 ?
4-methylmorpholine N-oxide + electron donor good substrate Escherichia coli 4-methylmorpholine + oxidized electron donor + H2O
-
 ?
alpha-picoline N-oxide + electron donor
-
 Escherichia coli alpha-picoline + H2O + oxidized electron donor
-
 ?
gamma-picoline N-oxide + electron donor
-
 Escherichia coli gamma-picoline + H2O + oxidized electron donor
-
 ?
hydroxylamine N-oxide + electron donor
-
 Escherichia coli hydroxylamine + H2O + oxidized electron donor
-
 ?
N,N-dimethyldodecylamine N-oxide + electron donor
-
 Escherichia coli N,N-dimethyldodecylamine + H2O + oxidized electron donor
-
 ?
N,N-dimethylhexylamine N-oxide + electron donor
-
 Escherichia coli N,N-dimethylhexylamine + oxidized electron donor + H2O
-
 ?
N-cyclohexyldimethylamine N-oxide + electron donor
-
 Escherichia coli N-cyclohexyldimethylamine + oxidized electron donor + H2O
-
 ?
pyridine N-oxide + electron donor
-
 Escherichia coli pyridine + oxidized electron donor + H2O
-
 ?
trimethylamine N-oxide + electron donor benzyl viologen as electron donor Escherichia coli trimethylamine + oxidized electron donor + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.8
-
 N,N-dimethylhexylamine N-oxide
-
 Escherichia coli
45.6
-
 N-cyclohexyldimethylamine N-oxide
-
 Escherichia coli
49.2
-
 3-hydroxypyridine N-oxide
-
 Escherichia coli
49.6
-
 gamma-picoline N-oxide
-
 Escherichia coli
53.5
-
 Pyridine N-oxide
-
 Escherichia coli
66.7
-
 4-chloropyridine N-oxide
-
 Escherichia coli
70
-
 hydroxylamine N-oxide
-
 Escherichia coli
96.5
-
 4-methylmorpholine N-oxide
-
 Escherichia coli
101
-
 2-chloropyridine N-oxide
-
 Escherichia coli
102
-
 alpha-picoline N-oxide
-
 Escherichia coli
137
-
 3-picoline N-oxide
-
 Escherichia coli
151
-
 Trimethylamine N-oxide
-
 Escherichia coli
155
-
 N,N-dimethyldodecylamine N-oxide
-
 Escherichia coli