Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | nonessential activator | Homo sapiens |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-dXMP | - |
Homo sapiens | |
6-Chloropurine ribonucleotide | - |
Homo sapiens | |
6-Thio-GMP | - |
Homo sapiens | |
6-thio-IMP | - |
Homo sapiens | |
6-Thio-XMP | - |
Homo sapiens | |
8-Aza-7-deaza-XMP | - |
Homo sapiens | |
8-Aza-XMP | - |
Homo sapiens | |
arabinosyl-XMP | - |
Homo sapiens | |
XMP | competitive vs. GMP, noncompetitive vs. NADPH | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
GMP | - |
Homo sapiens | |
0.0075 | - |
GMP | data from the low concentration range | Homo sapiens | |
0.0169 | - |
NADPH | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity purification on Cibacron blue | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + NH3 + NADP+ = GMP + NADPH + H+ | mechanism: ordered sequential with GMP binding first | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, 2-mercaptoethanol, 6 months, less than 15% loss of activity after several rounds of freezing and thawing | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + guanosine 5'-phosphate | less than 10% of the NADPH rate: thionicotinamide-NADPH, deamino-NADPH, 3-acetylpyrimidine-NADPH | Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
? | |
NADPH + guanosine 5'-phosphate | arabinosylGMP, 2'-dGMP and 8-azaGMP are reductively deaminated to their corresponding IMP analog at rates 1-2% the rate with GMP | Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no activity with NADH | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
XMP | - |
Homo sapiens | |
0.0003 | - |
8-Aza-XMP | - |
Homo sapiens | |
0.026 | - |
2'-dXMP | - |
Homo sapiens |