Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.6 extracted from

  • Ooi, T.; Ogata, D.; Matsumoto, K.; Nakamura, G.; Yu, J.; Yao, M.; Kitamura, M.; Taguchi, S.
    Flavin-binding of azoreductase: Direct evidences for dual-binding property of apo-azoreductase with FMN and FAD (2012), J. Mol. Catal. B, 74, 204-208.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
2 * 23000, SDS-PAGE Bacillus sp. (in: Bacteria)
45000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) B29
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.4
-
cofactor FAD, pH not specified in the publication, temperature not specified in the publication Bacillus sp. (in: Bacteria)
26.9
-
cofactor FMN, pH not specified in the publication, temperature not specified in the publication Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxidized methyl red + NADH + H+
-
Bacillus sp. (in: Bacteria) reduced methyl red + NAD+
-
?
oxidized methyl red + NADH + H+
-
Bacillus sp. (in: Bacteria) B29 reduced methyl red + NAD+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
AzrA
-
Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
FAD about 60% of the activity in presence of FMN. FAD binds to the apo-form and the bound FAD is stably retained in the enzyme molecule without degradation to FMN. The isoalloxazine ring of FAD localizes at the same site and plays the same role as that of FMN in the enzyme Bacillus sp. (in: Bacteria)
FMN
-
Bacillus sp. (in: Bacteria)