Cloned (Comment) | Organism |
---|---|
into the vector pET-11a for expression in Escherichia coli BL21-Gold DE3 pLysS cells | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
W105A | mutant, complete loss of both affinity for FMN and enzyme activity | Enterococcus faecalis |
W105F | mutant, lower Vmax value, decrease 30.6fold | Enterococcus faecalis |
W105G | mutant, complete loss of both affinity for FMN and enzyme activity | Enterococcus faecalis |
W105H | mutant, lower Vmax value, decrease 8.2fold | Enterococcus faecalis |
W105Q | mutant, lower Vmax value, decrease 68.2fold | Enterococcus faecalis |
W105Y | mutant, substitution does not significantly decrease the Vmax of the enzyme, 22% reduction | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0247 | - |
Methyl red | mutant W105F | Enterococcus faecalis | |
0.0251 | - |
Methyl red | mutant W105H | Enterococcus faecalis | |
0.0254 | - |
Methyl red | wild-type enzyme | Enterococcus faecalis | |
0.0276 | - |
Methyl red | mutant W105Y | Enterococcus faecalis | |
0.0282 | - |
Methyl red | mutant W105Q | Enterococcus faecalis | |
0.158 | - |
NADH | wild-type enzyme | Enterococcus faecalis | |
0.166 | - |
NADH | mutant W105Y | Enterococcus faecalis | |
0.169 | - |
NADH | mutant W105Q | Enterococcus faecalis | |
0.171 | - |
NADH | mutant W105H | Enterococcus faecalis | |
0.175 | - |
NADH | mutant W105F | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
monomer, determined by SDS-PAGE | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography and by gel filtration | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl red + NADH + H+ | - |
Enterococcus faecalis | ? + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 23000 | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
AzoA | - |
Enterococcus faecalis |
azoreductase | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
activity assay at room temperature | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
activity assay | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Enterococcus faecalis | |
NADH | - |
Enterococcus faecalis |