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Literature summary for 1.7.1.6 extracted from
- Purification and identification of an FMN-dependent NAD(P)H azoreductase from Enterococcus faecalis (2009), Curr. Issues Mol. Biol., 11, 59-65.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
FMN | - |
Enterococcus faecalis |  |
| 0.011 | - |
Methyl red | - |
Enterococcus faecalis | |
| 0.082 | - |
NADH | - |
Enterococcus faecalis | |
| 15 | - |
NADPH | - |
Enterococcus faecalis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | Q831B2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cell extracts of Enterococcus faecalis are prepared, AzoA is purified by hydrophobic, anion exchange and affinity chromatography | Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.003 | - |
purification step extract | Enterococcus faecalis |
| 0.014 | - |
purification octyl-sepharose 4 fast flow | Enterococcus faecalis |
| 0.224 | - |
purification DEAE Bio gel | Enterococcus faecalis |
| 0.626 | - |
purification step affinity gel | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl red + NAD(P)H + H+ | - |
Enterococcus faecalis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AzoA | - |
Enterococcus faecalis |
| azoreductase | - |
Enterococcus faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Enterococcus faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Enterococcus faecalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | FMN-dependent | Enterococcus faecalis | |
| NADH | 180fold preference for NADH over NADPH as an electron donor to reduce methyl red | Enterococcus faecalis | |
| NADPH | 180fold preference for NADH over NADPH as an electron donor to reduce methyl red | Enterococcus faecalis | |
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