Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold(DE3)pLysS | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | pH 7.1, 23°C | Enterococcus faecalis | |
0.14 | - |
NADH | pH 7.1, 23°C | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE | Enterococcus faecalis |
43000 | - |
gel filtration | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Enterococcus faecalis | important for the conversion of azo dyes in the gastrointestinal tract | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
strain ATCC 19433, predominant member of the human gastrointestinal microflora | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.9 | - |
purified enzyme, pH 7.1, 23°C, with Methyl red and NADH as substrates | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4'-carboxyphenylazo)-2-naphthol + NADH | substrate is carboxy-Orange II | Enterococcus faecalis | 4-amino-1-benzoic acid + NAD+ + 1-amino-2-hydroxynaphthalene | - |
? | |
2-[4-(dimethylamino)phenylazo]benzoic acid + NADH | substrate is Methyl red | Enterococcus faecalis | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? | |
Amaranth + NADH | - |
Enterococcus faecalis | ? | - |
? | |
additional information | important for the conversion of azo dyes in the gastrointestinal tract | Enterococcus faecalis | ? | - |
? | |
Orange G + NADH | - |
Enterococcus faecalis | ? | - |
? | |
Ponceau BS + NADH | - |
Enterococcus faecalis | ? | - |
? | |
Ponceau S + NADH | - |
Enterococcus faecalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one molecule per enzyme dimer | Enterococcus faecalis | |
NADH | - |
Enterococcus faecalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterococcus faecalis | calculated | - |
4.8 |