Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.6 extracted from

  • Yan, B.; Zhou, J.; Wang, J.; Du, C.; Hou, H.; Song, Z.; Bao, Y.
    Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737 (2004), FEMS Microbiol. Lett., 236, 129-136.
    View publication on PubMed
Search for more literature for 1.7.1.6

Application

Application Comment Organism
environmental protection potential for the treatment of azo dye contaminated wastewater Cereibacter sphaeroides

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Cereibacter sphaeroides
Mn2+
-
 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 2-[4-(dimethylamino)phenylazo]benzoic acid GST-fusion protein, pH 8.0, 40°C Cereibacter sphaeroides
0.42
-
 2-[4-(dimethylamino)phenylazo]benzoic acid native protein, pH 8.0, 40°C Cereibacter sphaeroides
2.1
-
 NADH GST-fusion protein, pH 8.0, 40°C Cereibacter sphaeroides
2.3
-
 NADH native protein, pH 8.0, 40°C Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates activity Cereibacter sphaeroides
Zn2+ stimulates activity Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18700
-
 SDS-PAGE, enzyme from host strain Cereibacter sphaeroides
44000
-
 SDS-PAGE, recombinant GST-fusion protein Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q8GKS3
-
-
Cereibacter sphaeroides AS1.1737 Q8GKS3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.65
-
 extract from host strain with Methyl red and NADH as substrates, pH 8.0, 40°C Cereibacter sphaeroides
1.1
-
 purified recombinant enzyme with Red dye III and NADH as substrates, pH 8.0, 40°C Cereibacter sphaeroides
8.5
-
 extract from Escherichia coli containing recombinant enzyme with Methyl red and NADH as substrates, pH 8.0, 40°C Cereibacter sphaeroides
12.6
-
 purified recombinant enzyme with Red dye II and NADH as substrates, pH 8.0, 40°C Cereibacter sphaeroides
20.3
-
 purified recombinant enzyme with Red dye I and NADH as substrates, pH 8.0, 40°C Cereibacter sphaeroides
78.9
-
 purified recombinant enzyme with Methyl red and NADH as substrates, pH 8.0, 40°C Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate is carboxy-Orange II Cereibacter sphaeroides 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate is carboxy-Orange II Cereibacter sphaeroides AS1.1737 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
2-[4-(dimethylamino)phenylazo]benzoic acid + NADH substrate is Methyl red Cereibacter sphaeroides 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+
-
 ?
2-[4-(dimethylamino)phenylazo]benzoic acid + NADH substrate is Methyl red Cereibacter sphaeroides AS1.1737 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+
-
 ?
Acid red G + NADH
-
 Cereibacter sphaeroides ?
-
 ?
Acid red G + NADH
-
 Cereibacter sphaeroides AS1.1737 ?
-
 ?
Methyl orange + NADH
-
 Cereibacter sphaeroides ?
-
 ?
Methyl orange + NADH
-
 Cereibacter sphaeroides AS1.1737 ?
-
 ?
Red dye I + NADH
-
 Cereibacter sphaeroides ?
-
 ?
Red dye I + NADH
-
 Cereibacter sphaeroides AS1.1737 ?
-
 ?
Red dye II + NADH
-
 Cereibacter sphaeroides ?
-
 ?
Red dye III + NADH
-
 Cereibacter sphaeroides ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 GST-fusion protein Cereibacter sphaeroides
50
-
 native protein Cereibacter sphaeroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90
-
 Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Cereibacter sphaeroides