Application | Comment | Organism |
---|---|---|
environmental protection | potential for the treatment of azo dye contaminated wastewater | Cereibacter sphaeroides |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Cereibacter sphaeroides | |
Mn2+ | - |
Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | GST-fusion protein, pH 8.0, 40°C | Cereibacter sphaeroides | |
0.42 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | native protein, pH 8.0, 40°C | Cereibacter sphaeroides | |
2.1 | - |
NADH | GST-fusion protein, pH 8.0, 40°C | Cereibacter sphaeroides | |
2.3 | - |
NADH | native protein, pH 8.0, 40°C | Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates activity | Cereibacter sphaeroides | |
Zn2+ | stimulates activity | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18700 | - |
SDS-PAGE, enzyme from host strain | Cereibacter sphaeroides |
44000 | - |
SDS-PAGE, recombinant GST-fusion protein | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | Q8GKS3 | - |
- |
Cereibacter sphaeroides AS1.1737 | Q8GKS3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.65 | - |
extract from host strain with Methyl red and NADH as substrates, pH 8.0, 40°C | Cereibacter sphaeroides |
1.1 | - |
purified recombinant enzyme with Red dye III and NADH as substrates, pH 8.0, 40°C | Cereibacter sphaeroides |
8.5 | - |
extract from Escherichia coli containing recombinant enzyme with Methyl red and NADH as substrates, pH 8.0, 40°C | Cereibacter sphaeroides |
12.6 | - |
purified recombinant enzyme with Red dye II and NADH as substrates, pH 8.0, 40°C | Cereibacter sphaeroides |
20.3 | - |
purified recombinant enzyme with Red dye I and NADH as substrates, pH 8.0, 40°C | Cereibacter sphaeroides |
78.9 | - |
purified recombinant enzyme with Methyl red and NADH as substrates, pH 8.0, 40°C | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | substrate is carboxy-Orange II | Cereibacter sphaeroides | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | substrate is carboxy-Orange II | Cereibacter sphaeroides AS1.1737 | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
2-[4-(dimethylamino)phenylazo]benzoic acid + NADH | substrate is Methyl red | Cereibacter sphaeroides | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? | |
2-[4-(dimethylamino)phenylazo]benzoic acid + NADH | substrate is Methyl red | Cereibacter sphaeroides AS1.1737 | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? | |
Acid red G + NADH | - |
Cereibacter sphaeroides | ? | - |
? | |
Acid red G + NADH | - |
Cereibacter sphaeroides AS1.1737 | ? | - |
? | |
Methyl orange + NADH | - |
Cereibacter sphaeroides | ? | - |
? | |
Methyl orange + NADH | - |
Cereibacter sphaeroides AS1.1737 | ? | - |
? | |
Red dye I + NADH | - |
Cereibacter sphaeroides | ? | - |
? | |
Red dye I + NADH | - |
Cereibacter sphaeroides AS1.1737 | ? | - |
? | |
Red dye II + NADH | - |
Cereibacter sphaeroides | ? | - |
? | |
Red dye III + NADH | - |
Cereibacter sphaeroides | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
GST-fusion protein | Cereibacter sphaeroides |
50 | - |
native protein | Cereibacter sphaeroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | - |
Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cereibacter sphaeroides |