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Literature summary for 1.7.1.6 extracted from
- Purification and characterization of an oxygen insensitive azoreductase from Pseudomonas aeruginosa (2005), Enzyme Microb. Technol., 36, 503-509.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | potential for the treatment of azo dye contaminated wastewater | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Pseudomonas aeruginosa |  |
| Fe2+ | complete inhibition | Pseudomonas aeruginosa | |
| Hg2+ | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
Navitan fast blue S5R | pH 7.0, room temperature | Pseudomonas aeruginosa | |
| 4.35 | - |
NADH | pH 7.0, room temperature | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
SDS-PAGE, native PAGE, gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Amaranth + NADH | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Amido black + NADH | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Atul acid black + NADH | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Eriochrome blue black + NADH | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Navitan fast blue S5R + NADH | - |
Pseudomonas aeruginosa | metanilic acid + 1,4-diamino naphtalene + peri acid + NAD+ | - |
? | |
| Orange G + NADH | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Tropaeolin + NADH | - |
Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, subunit mass by SDS-PAGE, native mass by gel filtration | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas aeruginosa | |
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