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Literature summary for 1.7.1.6 extracted from
- Purification and partial characterization of azoreductase from Enterobacter agglomerans (2003), Arch. Biochem. Biophys., 413, 139-146.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | potential for the treatment of azo dye contaminated wastewater | Pantoea agglomerans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | IC50: 0.13 mM | Pantoea agglomerans |  |
| Fe2+ | IC50: 0.11 mM | Pantoea agglomerans | |
| Hg2+ | IC50: 0.17 mM | Pantoea agglomerans | |
| Mn2+ | IC50: 0.43 mM | Pantoea agglomerans | |
| Zn2+ | IC50: 0.28 mM | Pantoea agglomerans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | pH 7.0, 25°C | Pantoea agglomerans | |
| 0.059 | - |
NADH | pH 7.0, 25°C | Pantoea agglomerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
native PAGE | Pantoea agglomerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pantoea agglomerans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pantoea agglomerans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol | Pantoea agglomerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-[4-(dimethylamino)phenylazo]benzoic acid + NADH | substrate is Methyl red | Pantoea agglomerans | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? | |
| Amaranth + NADH | 35% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
| Disperse yellow + NADH | 85% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
| Orange G + NADH | 30% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
| Trypan blue + NADH | 50% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Pantoea agglomerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pantoea agglomerans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 60 | - |
Pantoea agglomerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pantoea agglomerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Pantoea agglomerans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pantoea agglomerans | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
IC50: 0.11 mM | Pantoea agglomerans | Fe2+ | |
| 0.13 | - |
IC50: 0.13 mM | Pantoea agglomerans | Cd2+ | |
| 0.17 | - |
IC50: 0.17 mM | Pantoea agglomerans | Hg2+ | |
| 0.28 | - |
IC50: 0.28 mM | Pantoea agglomerans | Zn2+ | |
| 0.43 | - |
IC50: 0.43 mM | Pantoea agglomerans | Mn2+ | |
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