Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, recombinant and native enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
sp. OY1-2 isolated from soil near a waste-water plant | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
recombinant enzyme at 50°C with roccelin | Bacillus sp. (in: Bacteria) |
11.7 | - |
native enzyme at 70°C with roccelin | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Rocceline + NADPH | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
Solar Orange + NADPH | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
Sumifix Black B + NADPH | - |
Bacillus sp. (in: Bacteria) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, recombinant and native enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |
70 | - |
native enzyme | Bacillus sp. (in: Bacteria) |