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Literature summary for 1.7.1.4 extracted from
- Reduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum (1973), Biochem. J., 133, 701-708.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | no effect | Azotobacter chroococcum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Carbamoyl phosphate | - |
Azotobacter chroococcum | |
| CN- | 0.01 mM, 40% inhibition, 0.1 mM, 93% inhibition | Azotobacter chroococcum | |
| CO | competitive vs. nitrite | Azotobacter chroococcum | |
| Cyanate | - |
Azotobacter chroococcum | |
| NAD(P)H | inactivation after preincubation, presence of nitrite protects from inactivation | Azotobacter chroococcum | |
| NaN3 | - |
Azotobacter chroococcum | |
| p-hydroxymercuribenzoate | 0.01 mM, 42% inhibition, 0.1 mM, complete inhibition | Azotobacter chroococcum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0055 | - |
NO2- | - |
Azotobacter chroococcum | |
| 0.015 | - |
NADH | - |
Azotobacter chroococcum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
sucrose density gradient centrifugation | Azotobacter chroococcum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter chroococcum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD(P)H + nitrite + H+ | NADH is preferred, S2O42- can act as electron donor, methyl viologen as electron carrier | Azotobacter chroococcum | NAD(P)+ + NH3 + H2O | - |
? | |
| nitrite + NADH + H+ | - |
Azotobacter chroococcum | NH3 + NAD+ + H2O | - |
r | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | stimulation | Azotobacter chroococcum | |
| additional information | FMN has no effect | Azotobacter chroococcum | |
| NADH | - |
Azotobacter chroococcum | |
| NADPH | - |
Azotobacter chroococcum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000032 | - |
CO | - |
Azotobacter chroococcum | |
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