General Stability | Organism |
---|---|
dialysis for 15 h against 1 mM cyanide, 1 mM 8-hydroxyquinoline or 1 mM EDTA followed by dialysis against 0.05 M phosphate buffer, no decrease in activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | 0.5 mM, 62% inhibition | Escherichia coli | |
CN- | 0.02 mM, 88% inhibition | Escherichia coli | |
NADP+ | 0.06 mM, 13% inhibition, 0.27 mM, 43% inhibition | Escherichia coli | |
p-chloromercuribenzoate | 0.001 mM, 83% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
NO2- | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli Bn | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + nitrite + H+ | specific for NADH | Escherichia coli | NAD(P)+ + NH3 + H2O | - |
? | |
NAD(P)H + nitrite + H+ | specific for NADH | Escherichia coli Bn | NAD(P)+ + NH3 + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 50% activity, pH 9.5: about 15% activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme is specific for NADPH, NADH reaction rate approx. 1% of NADPH rate | Escherichia coli | |
NADPH | enzyme is specific for NADPH, NADH reaction rate approx. 1% of NADPH rate | Escherichia coli |