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Literature summary for 1.7.1.3 extracted from
- Aerobic and anaerobic nitrate and nitrite reduction in free-living cells of Bradyrhizobium sp. (Lupinus) (2003), FEMS Microbiol. Lett., 226, 331-337.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | growth stimulation is higher on nitrate than on nitrite | Bradyrhizobium sp. | |
| NH4+ | slight activation of the enzyme under anaerobic conditions | Bradyrhizobium sp. |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | intracellularly accumulated nitrite can inhibit nitrate uptake, addition of nitrite at concentrations above 5 mM is toxic and causes growth retardation | Bradyrhizobium sp. | |
| NH4+ | inhibits the enzyme under aerobic conditions | Bradyrhizobium sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bradyrhizobium sp. | - |
Lupinus, strain USDA 3045 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | free-living cells, yeast extract/mannitol medium | Bradyrhizobium sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
activity under aerobic conditions in presence of NH4+ | Bradyrhizobium sp. |
| 0.1 | - |
activity under aerobic conditions | Bradyrhizobium sp. |
| 0.3 | - |
activity under anaerobic conditions | Bradyrhizobium sp. |
| 0.37 | - |
activity under anaerobic conditions in presence of NH4+ | Bradyrhizobium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + reduced benzyl viologen | - |
Bradyrhizobium sp. | nitrite + benzyl viologen | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrate reductase | - |
Bradyrhizobium sp. |
| NR | - |
Bradyrhizobium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
a growth temperature above 20°C is required | Bradyrhizobium sp. |
| 30 | - |
assay at | Bradyrhizobium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bradyrhizobium sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| benzyl viologen | - |
Bradyrhizobium sp. | |
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