Protein Variants | Comment | Organism |
---|---|---|
additional information | nit-3 mutant (FGSC 262): reduced FAD-nitrate reductase and reduced methylviologen-nitrate reductase activities | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | at 0.1 mM complete inhibition | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
235000 | - |
sucrose density gradient centrifugation, gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
wild-type STA4, FGSC 358 and nit-1 mutant, FGSC 262 | - |
Purification (Comment) | Organism |
---|---|
partially purified | Neurospora crassa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
nitrite + NADP+ + H2O = nitrate + NADPH + H+ | sulfhydryl groups may participate in the binding of the protein subunits | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.55 | - |
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
-20°C, stable for 6 months or longer, 5% loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | associated FAD-nitrate reductase and methylviologen-nitrate activity | Neurospora crassa | nitrite + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | at least 2 subunits suggested | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
labile, loss of acitvity after 2 min | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
optima of associated activities | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b557 | - |
Neurospora crassa | |
FAD | - |
Neurospora crassa |