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Literature summary for 1.7.1.14 extracted from

  • Kaya, M.; Matsumura, K.; Higashida, K.; Hata, Y.; Kawato, A.; Abe, Y.; Akita, O.; Takaya, N.; Shoun, H.
    Cloning and enhanced expression of the cytochrome P450nor gene (nicA; CYP55A5) encoding nitric oxide reductase from Aspergillus oryzae (2004), Biosci. Biotechnol. Biochem., 68, 2040-2049.
    View publication on PubMed
Search for more literature for 1.7.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.801
-
 NADH in 50 mM sodium phosphate (pH 7.2), at 37°C Aspergillus oryzae
0.934
-
 NADPH in 50 mM sodium phosphate (pH 7.2), at 37°C Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Aspergillus oryzae
45690
-
 deduced from amino acid sequence Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q8NKB4
-
-
Aspergillus oryzae OSI 1013 Q8NKB4
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Resource Q column chromatography, Resource PHE column chromatography, and Superdex 200 gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NO + NADH + H+
-
 Aspergillus oryzae N2O + NAD+ + H2O
-
 ?
NO + NADH + H+
-
 Aspergillus oryzae OSI 1013 N2O + NAD+ + H2O
-
 ?
NO + NADPH + H+
-
 Aspergillus oryzae N2O + NADP+ + H2O
-
 ?
NO + NADPH + H+
-
 Aspergillus oryzae OSI 1013 N2O + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Anor
-
 Aspergillus oryzae
cytochrome P450nor
-
 Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
heme
-
 Aspergillus oryzae
NADH the enzyme employs both NADH and NADPH as electron donors Aspergillus oryzae
NADPH the enzyme employs both NADH and NADPH as electron donors Aspergillus oryzae