General Stability | Organism |
---|---|
dialysis against 3 mM sodium diphosphate, 1 mM cysteine solution, pH 8, 2 h, less than 50% loss of activity, against 10 mM phosphate, pH 8, 1 h, complete loss of activity | Neurospora crassa |
little or no activity in phosphate buffer unless 1 mM EDTA is included in the reaction mixture | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Neurospora crassa | |
8-hydroxyquinoline | - |
Neurospora crassa | |
Cu2+ | - |
Neurospora crassa | |
KCN | 0.1 mM, complete inhibition | Neurospora crassa | |
NaN3 | - |
Neurospora crassa | |
o-phenanthroline | - |
Neurospora crassa | |
potassium ethyl xanthate | - |
Neurospora crassa | |
Salicylaldoxime | - |
Neurospora crassa | |
Sodium diethyl dithiocarbamate | - |
Neurospora crassa | |
sulfite | - |
Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADH | - |
Neurospora crassa | |
0.1 | - |
NADPH | - |
Neurospora crassa | |
3.8 | - |
hydroxylamine | - |
Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
360.0 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute | Neurospora crassa |
Storage Stability | Organism |
---|---|
-15°C, slow loss of activity over several weeks | Neurospora crassa |
4°C, overnight | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADH | no other substances beside nitrite and dinitrobenzene stimulate oxidation of NADPH | Neurospora crassa | NH3 + NAD+ + H2O | - |
ir | |
hydroxylamine + NADH | no activity with O-methylhydroxylamine, hydrazine, nitrophenylhydroxylamine and salicylhydroxamic acid | Neurospora crassa | NH3 + NAD+ + H2O | - |
ir | |
hydroxylamine + NADPH + H+ | - |
Neurospora crassa | NH3 + NADP+ + H2O | - |
r | |
NO2- + NADH | - |
Neurospora crassa | NH3 + H2O + NAD+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, stable | Neurospora crassa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | highest stability | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | stimulates | Neurospora crassa | |
NADH | - |
Neurospora crassa | |
NADPH | - |
Neurospora crassa |