Literature summary for 1.7.1.10 extracted from

Roussos, G.G.; Nason, A.
Pyridine nucleotide-nitrite and -hydroxylamine enzymes from soybean leaves (1960), J. Biol. Chem., 235, 2997-3007.

Search for more literature for 1.7.1.10

Inhibitors

Inhibitors	Comment	Organism	Structure
2,2'-dipyridyl	1 mM, 84% inhibition	Glycine max
8-hydroxyquinoline	1 mM, complete inhibition	Glycine max
hydrazine	1 mM, complete inhibition	Glycine max
KCN	0.1 mM, complete inhibition	Glycine max
NaN3	1 mM, complete inhibition	Glycine max
o-phenanthroline	1 mM, complete inhibition	Glycine max
p-chloromercuribenzoate	0.1 mM, complete inhibition	Glycine max
potassium ethyl xanthate	-	Glycine max
Salicylaldoxime	-	Glycine max
Sodium diethyl dithiocarbamate	1 mM, complete inhibition	Glycine max

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Glycine max	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	0.1 mM, approx. 6fold stimulation	Glycine max

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	soybean	-

Purification (Commentary)

Purification (Comment)	Organism
enzyme not seperated from pyridine nucleotide-nitrite reductase	Glycine max

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Glycine max	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	794.1 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute	Glycine max

Storage Stability

Storage Stability	Organism
-20°C, 1 month, 60-90% loss of activity	Glycine max
4°C, 1 week, no loss in activity	Glycine max

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxylamine + NADH	NADH is 1.5fold more effective than NADPH	Glycine max	NH3 + NAD+ + H2O	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	complete loss of activity after 5 min	Glycine max

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	7.1	-	Glycine max

Cofactor

Cofactor	Comment	Organism	Structure
additional information	purified enzyme: absolute requirement for an unidentified, dissociable, heat-stable, organic factor obtained from soybean leaf extract, no flavin requirement	Glycine max
NADH	-	Glycine max
NADPH	about 65% of the activity with NADH	Glycine max

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Release 2023.1 (January 2023)