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Literature summary for 1.7.1.1 extracted from

  • Lambeck, I.; Chi, J.C.; Krizowski, S.; Mueller, S.; Mehlmer, N.; Teige, M.; Fischer, K.; Schwarz, G.
    Kinetic analysis of 14-3-3-inhibited Arabidopsis thaliana nitrate reductase (2010), Biochemistry, 49, 8177-8186.
    View publication on PubMed
Search for more literature for 1.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
mutant S534A is expressed in Pichia pastoris strainKM71 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
S534A the mutant is not inhibited by 14-3-3 proteins Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
14-3-3 protein isoform chi noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform epsilon noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform kappa noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform lambda noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform ni noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform omega noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform phi noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana
14-3-3 protein isoform theta noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.197
-
 nitrate 22°C, pH not specified in the publication Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADH + H+ Arabidopsis thaliana
-
 nitrite + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
SourceQ15 column chromatography and Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH + H+
-
 Arabidopsis thaliana nitrite + NAD+ + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
 nitrate 22°C, pH not specified in the publication Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
heme
-
 Arabidopsis thaliana
NADH
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00006
-
 14-3-3 protein isoform omega 22°C, pH not specified in the publication Arabidopsis thaliana
0.00008
-
 14-3-3 protein isoform lambda 22°C, pH not specified in the publication Arabidopsis thaliana
0.00014
-
 14-3-3 protein isoform kappa 22°C, pH not specified in the publication Arabidopsis thaliana
0.00023
-
 14-3-3 protein isoform ni 22°C, pH not specified in the publication Arabidopsis thaliana
0.00031
-
 14-3-3 protein isoform phi 22°C, pH not specified in the publication Arabidopsis thaliana
0.00034
-
 14-3-3 protein isoform chi 22°C, pH not specified in the publication Arabidopsis thaliana
0.00076
-
 14-3-3 protein isoform theta 22°C, pH not specified in the publication Arabidopsis thaliana