Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 0.1-5 mM, strong, both cytosolic and membrane-bound isozyme | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
NADH | pH 7.5, 30°C, membrane-bound isoform | Solanum lycopersicum | |
0.048 | - |
NADH | pH 7.5, 30°C, cytosolic isoform | Solanum lycopersicum | |
0.27 | - |
nitrate | pH 7.5, 30°C, cytosolic isoform | Solanum lycopersicum | |
0.97 | - |
nitrate | pH 7.5, 30°C, membrane-bound isoform | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | two isozymes, a membranebound, and a cytosolic form | Solanum lycopersicum | 5829 | - |
membrane | two isozymes, a membranebound, and a cytosolic form | Solanum lycopersicum | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
cv. Mill, membrane-bound isozyme remains more active in the dark and is more easily recovered after transfer to light than cytosolic isoform, inhibition of both forms by saline stress, cytosolic form is strongly induced and strictly dependent on presence of nitrate | - |
Purification (Comment) | Organism |
---|---|
extracting strength of different detergents on membrane-bound isozyme | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + nitrate + H+ | - |
Solanum lycopersicum | NAD+ + nitrite + H2O | - |
? |