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Literature summary for 1.7.1.1 extracted from

  • Trimboli, A.J.; Quinn, G.B.; Smith, E.T.; Barber, M.J.
    Thiol modification and site directed mutagenesis of the flavin domain of spinach NADH:nitrate reductase (1996), Arch. Biochem. Biophys., 331, 117-126.
    View publication on PubMed
Search for more literature for 1.7.1.1

Protein Variants

Protein Variants Comment Organism
C17S visible and CD spectra are very similar to that of the wild-type domain, thermal stability is slightly decreased compared to the wild-type domain Spinacia oleracea
C240A decrease of diaphorase activity Spinacia oleracea
C240G decrease of diaphorase activity Spinacia oleracea
C240S decrease of diaphorase activity, thermal stability is slightly decreased compared to the wild-type domain, the oxidation reduction midpoint potential for the FAD/FADH2 couple is -219 mV compared to -268 mV in the wild-type domain Spinacia oleracea
C54S visible and CD spectra are very similar to that of the wild-type domain, thermal stability is slightly decreased compared to the wild-type domain,the oxidation reduction midpoint potential for the FAD/FADH2 couple is -197 mV compared to -268 mV in the wild-type domain Spinacia oleracea
C62S visible and CD spectra are very similar to that of the wild-type domain, thermal stability is decreased compared to the wild-type domain, the oxidation reduction midpoint potential for the FAD/FADH2 couple is -226 mV compared to -268 mV in the wild-type domain Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid
-
 Chlorella sp.
2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid
-
 Spinacia oleracea
methyl methanethiosulfonate
-
 Chlorella sp.
methyl methanethiosulfonate inactivation is concentration independent Spinacia oleracea
NAD+
-
 Spinacia oleracea
NEM
-
 Chlorella sp.
NEM
-
 Spinacia oleracea
PCMB
-
 Chlorella sp.
PCMB inactivation is concentration independent Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 NADH recombinant flavin domain of nitrate reductase C240A mutant Spinacia oleracea
0.011
-
 NADH recombinant flavin domain of nitrate reductase C240G mutant Spinacia oleracea
0.012
-
 NADH wild-type recombinant flavin domain of nitrate reductase Spinacia oleracea
0.014
-
 NADH recombinant flavin domain of nitrate reductase C240S mutant Spinacia oleracea
0.015
-
 NADH recombinant flavin domain of nitrate reductase C17S mutant Spinacia oleracea
0.015
-
 NADH recombinant flavin domain of nitrate reductase C54S mutant Spinacia oleracea
0.016
-
 NADH recombinant flavin domain of nitrate reductase C62S mutant Spinacia oleracea
0.024
-
 NADH recombinant flavin domain of nitrate reductase C240S mutant Spinacia oleracea
0.027
-
 ferricyanide wild-type recombinant flavin domain of nitrate reductase Spinacia oleracea
0.036
-
 ferricyanide recombinant flavin domain of nitrate reductase C54S mutant Spinacia oleracea
0.045
-
 ferricyanide recombinant flavin domain of nitrate reductase C17S mutant Spinacia oleracea
0.059
-
 NADH recombinant flavin domain of nitrate reductase C62S mutant Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Chlorella sp.
-
-
-
Spinacia oleracea
-
 recombinant flavin domain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Spinacia oleracea 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricyanide + NADH
-
 Chlorella sp. 2 ferrocyanide + NAD+ + H+
-
 ?
nitrate + NADH
-
 Spinacia oleracea nitrite + NAD+ + H2O
-
 ?
nitrate + NADH
-
 Chlorella sp. nitrite + NAD+ + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Spinacia oleracea
NADH
-
 Chlorella sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 NAD+ recombinant flavin domain of nitrate reductase C240G mutant Spinacia oleracea
0.8
-
 NAD+ recombinant flavin domain of nitrate reductase C240A mutant Spinacia oleracea
1.2
-
 NAD+ recombinant flavin domain of nitrate reductase C240S mutant Spinacia oleracea
1.5
-
 NAD+ recombinant flavin domain of nitrate reductase C54S mutant Spinacia oleracea
1.9
-
 NAD+ recombinant flavin domain of nitrate reductase wild-type and C17S mutant Spinacia oleracea
2
-
 NAD+ recombinant flavin domain of nitrate reductase C62S mutant Spinacia oleracea