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Literature summary for 1.7.1.1 extracted from
- Purification and characterization of NADH-nitrate reductase from leaves of 2-row barley, and its activity as affected by some metabolites (1985), J. Plant Physiol., 119, 247-256.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| HPO42- | activates | Hordeum vulgare |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive with ADP | Hordeum vulgare | |
| Carbamoyl phosphate | competitive | Hordeum vulgare | |
| Co2+ | 1 mM, strong inhibition | Hordeum vulgare | |
| Cu2+ | 1 mM, strong inhibition | Hordeum vulgare | |
| Mn2+ | 1 mM, 27% inhibition | Hordeum vulgare | |
| MoO42- | 1 mM, strong inhibition | Hordeum vulgare | |
| NO2- | 10 mM, 65% inhibition | Hordeum vulgare | |
| pyridoxal 5'-phosphate | uncompetitive with nitrate | Hordeum vulgare | |
| VO3- | 1 mM, strong inhibition | Hordeum vulgare | |
| Zn2+ | 1 mM, strong inhibition | Hordeum vulgare | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
NADH | - |
Hordeum vulgare | |
| 0.27 | - |
nitrate | - |
Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 225000 | - |
disc gel electrophoresis | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADH | - |
Hordeum vulgare | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 2 ferricytochrome c + NADH | - |
Hordeum vulgare | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| FADH2 + nitrate | - |
Hordeum vulgare | FAD + nitrite | - |
? | |
| FMNH2 + nitrate | - |
Hordeum vulgare | FMN + nitrite | - |
? | |
| nitrate + NADH | - |
Hordeum vulgare | nitrite + NAD+ + H2O | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Hordeum vulgare | ? + NAD+ | - |
? | |
| reduced methyl viologen + nitrate | - |
Hordeum vulgare | methyl viologen + nitrite | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
pH 7.5, half-life: 42 h | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Hordeum vulgare |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
0°C, half-life: 42 h | Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | required for maximal activity, Km: 8 nM | Hordeum vulgare | |
| NADH | - |
Hordeum vulgare | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
Carbamoyl phosphate | - |
Hordeum vulgare | |
| 0.176 | - |
pyridoxal 5'-phosphate | - |
Hordeum vulgare | |
| 1.36 | - |
ADP | - |
Hordeum vulgare | |
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