Cloned (Comment) | Organism |
---|---|
overexpression of the enzyme in Escherichia coli | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in a ternary complex with inhibitor 12-oxophytodienoate and FMN, X-ray diffraction structure determination and analysis at 2.05 A resolution | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(9R,13R)-12-oxophytodienoate | - |
Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma cruzi | old yellow enzyme is an NADPH oxidoreductase capable of reducing a variety of compounds, it catalyzes one- or two-electron reduction | ? | - |
? | |
NADPH + H+ + acceptor | Trypanosoma cruzi | - |
NADP+ + reduced acceptor | - |
? | |
NADPH + H+ + prostaglandin H2 | Trypanosoma cruzi | via FMN cofactor, stereoselective reaction mechanism | NADP+ + prostaglandin F2alpha | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NADPH + H+ + acceptor = NADP+ + reduced acceptor | reaction mechanism involving a conserved triad of Glu193-His284-Arg249, overview | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | old yellow enzyme is an NADPH oxidoreductase capable of reducing a variety of compounds, it catalyzes one- or two-electron reduction | Trypanosoma cruzi | ? | - |
? | |
NADPH + H+ + 4-hydroxybenzaldehyde | - |
Trypanosoma cruzi | NADP+ + ? | - |
? | |
NADPH + H+ + acceptor | - |
Trypanosoma cruzi | NADP+ + reduced acceptor | - |
? | |
NADPH + H+ + prostaglandin H2 | via FMN cofactor, stereoselective reaction mechanism | Trypanosoma cruzi | NADP+ + prostaglandin F2alpha | - |
? |
Synonyms | Comment | Organism |
---|---|---|
old yellow enzyme | - |
Trypanosoma cruzi |
OYE | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | prosthetic group | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
(9R,13R)-12-oxophytodienoate | pH 7.0, 25°C | Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
additional information | structure modelling of the enzyme with bound substrate prostaglandin H2 | Trypanosoma cruzi |