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Literature summary for 1.6.5.5 extracted from
- Oxidative-stress-inducible qorA encodes an NADPH-dependent quinone oxidoreductase catalysing a one-electron reduction in Staphylococcus aureus (2003), Microbiology, 149, 389-398.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0491 | - |
9,10-phenanthrenequinone | pH 7.5, 25°C | Staphylococcus aureus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36300 | - |
x * 36300, calculated and co-purification results | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | A0A0H3JVE7 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.8 | - |
substrate menadione, pH 7.5, 25°C | Staphylococcus aureus |
| 311 | - |
substrate 9,10-phenanthrenequinone, pH 7.5, 25°C | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9,10-phenanthrenequinone + NADPH + H+ | one-electron reduction mechanism. Concomitantly with NADPH consumption, generation of superoxide is observed | Staphylococcus aureus | 9,10-phenanthrenequinol + NADP+ | - |
? | |
| menadione + NADPH + H+ | - |
Staphylococcus aureus | menadiol + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 36300, calculated and co-purification results | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| QorA | - |
Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor: NADH. Sequence does not contain a flavin-binding motif | Staphylococcus aureus | |
| NADPH | - |
Staphylococcus aureus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Staphylococcus aureus | calculated | - |
5.2 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Staphylococcus aureus | induced in presence of 9,10-phenanthrenequinone and by oxidative stress | up |
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Release 2023.1 (January 2023)
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