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Literature summary for 1.6.5.5 extracted from
- Purification and characterization of zeta-crystallin from the camel lens (1995), Biochem. Biophys. Res. Commun., 215, 632-640.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Camelus dromedarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0069 | - |
NADPH | - |
Camelus dromedarius |  |
| 0.017 | - |
9,10-phenanthrenequinone | - |
Camelus dromedarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
4 * 35000, SDS-PAGE | Camelus dromedarius |
| 140000 | - |
gel filtration | Camelus dromedarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
camel | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lens | cortex | Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.1 | - |
- |
Camelus dromedarius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5 months, stable | Camelus dromedarius |
| 4°C, stable for at least 1 month | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-naphthoquinone + NADPH | - |
Camelus dromedarius | 1,2-naphthoquinol + NADP+ | - |
? | |
| 1,4-benzoquinone + NADPH | - |
Camelus dromedarius | 1,4-benzosemiquinone + NADP+ | - |
? | |
| 1,4-naphthoquinone + NADPH + H+ | - |
Camelus dromedarius | 1,4-naphthosemiquinone + NADP+ | - |
? | |
| 9,10-phenanthrenequinone + NADPH | - |
Camelus dromedarius | 9,10-phenanthrenesemiquinone + NADP+ | - |
? | |
| additional information | no activity with menadione and 9,10-anthraquinone | Camelus dromedarius | ? | - |
? | |
| NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Camelus dromedarius | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000, SDS-PAGE | Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Camelus dromedarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | about 60% of maximal activity at 20°C and at 60°C | Camelus dromedarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Camelus dromedarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | pH 4.5: about 60% of maximal activity, pH 10: about 50% of maximal activity | Camelus dromedarius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Camelus dromedarius | |
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