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Literature summary for 1.6.5.5 extracted from

  • Rao, P.V.; Krishna, C.M.; Zigler, J.S.
    Identification and characterization of the enzymatic activity of zeta-Crystallin from guinea pig lens (1992), J. Biol. Chem., 267, 96-102.
    View publication on PubMed
Search for more literature for 1.6.5.5

General Stability

General Stability Organism
NADPH protects against inactivation caused by heat, NEM or H2O2 Cavia porcellus
very stable to freezing Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Cavia porcellus
Cibacron blue 3GA
-
 Cavia porcellus
dicoumarol
-
 Cavia porcellus
H2O2
-
 Cavia porcellus
additional information no inactivation by 0.5 mM iodoacetate Cavia porcellus
NADP+
-
 Cavia porcellus
NEM
-
 Cavia porcellus
Nitrofurantoin
-
 Cavia porcellus
p-chloromercuriphenylsulfonate
-
 Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 NADPH
-
 Cavia porcellus
0.01
-
 9,10-phenanthrenequinone
-
 Cavia porcellus
0.027
-
 5-hydroxy-1,4-naphthoquinone
-
 Cavia porcellus
0.143
-
 1,4-benzoquinone
-
 Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
NADPH + H+ + 2 quinone = NADP+ + 2 semiquinone enzyme acts through a one-electron transfer process Cavia porcellus
a

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
 Cavia porcellus
-

Storage Stability

Storage Stability Organism
-20°C, less than 10% loss of activity after 4-6 weeks Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-naphthoquinone + NADPH
-
 Cavia porcellus 1,2-naphthoquinol + NADP+
-
 ?
1,4-benzoquinone + NADPH 15.3% of the activity with 1,2-naphthoquinone Cavia porcellus 1,4-benzosemiquinone + NADP+
-
 ?
1,4-naphthoquinone + NADPH + H+ 2.6% of the activity with 1,2-naphthoquinone Cavia porcellus 1,4-naphthosemiquinone + NADP+
-
 ?
5-hydroxy-1,4-naphthoquinone + NADPH i.e. juglone, 12.5% of the activity with 1,2-naphthoquinone Cavia porcellus 5-hydroxy-1,4-naphthosemiquinone + NADP+
-
 ?
5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH i.e. plumbagin, 0.9% of the activity with 1,2-naphthoquinone Cavia porcellus 5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+
-
 ?
9,10-phenanthrenequinone + NADPH 50% of the activity with 1,2-naphthoquinone Cavia porcellus 9,10-phenanthrenesemiquinone + NADP+
-
 ?
methyl-1,4-benzoquinone + NADPH 20.6% of the activity with 1,2-naphthoquinone Cavia porcellus methyl-1,4-benzoquinol + NADP+
-
 ?
additional information inactive with: menadione, ubiquinone, 9,10-anthraquinone, vitamin K1, vitamin K2 Cavia porcellus ?
-
 ?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol 3.8% of the activity with 1,2-naphthoquinone Cavia porcellus NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 65% loss of activity Cavia porcellus
51
-
 10 min, complete loss of activity Cavia porcellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 5-hydroxy-1,4-naphthoquinone
-
 Cavia porcellus
3.4
-
 1,4-benzoquinone
-
 Cavia porcellus
10
-
 9,10-phenanthrenequinone
-
 Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Cavia porcellus