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Literature summary for 1.6.5.4 extracted from

  • Huang, G.; Deng, J.; Chen, H.; Huang, S.; Shih, C.; Lin, Y.
    Dehydroascorbate reductase and monodehydroascorbate reductase activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. Tainong 57) storage roots (2013), Bot. Stud., 54, 007 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
glutathione in the absene of glutathione, only negligible activity is found Ipomoea batatas

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas B6VJL9
-
-
Ipomoea batatas Q9ZSC6
-
-
Ipomoea batatas Lam. Tainong 57 B6VJL9
-
-
Ipomoea batatas Lam. Tainong 57 Q9ZSC6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Ipomoea batatas
-
leaf vein
-
Ipomoea batatas
-
leaf vein low expression Ipomoea batatas
-
root highest expression in storage root, followed by sprouted root Ipomoea batatas
-
root highest expression in storage root, low expression in sprouted root Ipomoea batatas
-
sprout low expression Ipomoea batatas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00345
-
presence of glutathione, pH 7.0, temperature not specified in the publication Ipomoea batatas
0.00552
-
presence of glutathione, pH 7.0, temperature not specified in the publication Ipomoea batatas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monodehydroascorbate + NADH + H+
-
Ipomoea batatas ascorbate + NAD+
-
?
monodehydroascorbate + NADH + H+
-
Ipomoea batatas Lam. Tainong 57 ascorbate + NAD+
-
?
additional information metallothionein-lke protein MT1K shows both dehydroascorbate reductase EC 1.8.5.1, and monodehydroascorbate reductase activity Ipomoea batatas ?
-
?
additional information metallothionein-lke protein MT1K shows both dehydroascorbate reductase EC 1.8.5.1, and monodehydroascorbate reductase activity Ipomoea batatas Lam. Tainong 57 ?
-
?

Subunits

Subunits Comment Organism
? x * 6614, calculated, x * 6500, SDS-PAGE Ipomoea batatas
? x * 8068, calculated, x * 8000, SDS-PAGE Ipomoea batatas

Synonyms

Synonyms Comment Organism
metallothionein-like type 1 protein
-
Ipomoea batatas
MT-2
-
Ipomoea batatas
MT1K
-
Ipomoea batatas

pI Value

Organism Comment pI Value Maximum pI Value
Ipomoea batatas calculated
-
4.6
Ipomoea batatas calculated
-
4.8