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Literature summary for 1.6.5.2 extracted from

  • Ian Menz, R.; Day, D.
    Purification and characterization of a 43-kDa rotenone-insensitive NADH dehydrogenase from plant mitochondria (1996), J. Biol. Chem., 271, 23117-23120.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 74% residual activity at 0.15 mM Beta vulgaris
ADP 86% residual activity at 0.2 mM Beta vulgaris
ATP 91% residual activity at 0.2 mM Beta vulgaris
dicumarol most potent inhibitor, 12% residual activity at 0.15 mM Beta vulgaris
EGTA 95% residual activity at 5 mM Beta vulgaris
Mersalyl 68% residual activity at 0.15 mM Beta vulgaris
additional information insensitive to rotenone, Ca2+, platanetin and flavone have no effect on the NADH:2,3-dimethoxy-5-methyl-1,4-benzoquinone reductase activity of the purified enzyme Beta vulgaris
NAD+ 96% residual activity at 0.2 mM Beta vulgaris
NADP+ 95% residual activity at 0.2 mM Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
ubiquinone-0 in 10 mM bis-Tris-propane buffer (pH 7.2), at 25°C Beta vulgaris
0.34
-
NADH in 10 mM bis-Tris-propane buffer (pH 7.2), at 25°C Beta vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Beta vulgaris 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Beta vulgaris
86000
-
gel filtration Beta vulgaris

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
blue affinity column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Beta vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Beta vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH 60% activity compared to the reactions of NADPH or NADH with 2,3-dimethoxy-5-methyl-1,4-benzoquinone Beta vulgaris 2 ferrocyanide + NAD+ + H+
-
?
deamino-NADH + H+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinone 45% activity compared to NADPH or NADH Beta vulgaris deamino-NAD+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
?
NADH + H+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
Beta vulgaris NAD+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
?
NADH + H+ + ubiquinone-0
-
Beta vulgaris NAD+ + ubiquinol-0
-
?
NADPH + H+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinone 100% activity Beta vulgaris NADP+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 43000, SDS-PAGE Beta vulgaris

Synonyms

Synonyms Comment Organism
Internal NADH dehydrogenase
-
Beta vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Beta vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 the enzyme maintains greater than 90% of its maximal activity between pH 5.5 and 7.5 Beta vulgaris

Cofactor

Cofactor Comment Organism Structure
FAD
-
Beta vulgaris
NADH
-
Beta vulgaris
NADPH
-
Beta vulgaris