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Literature summary for 1.6.5.2 extracted from

  • Rescigno, A.; Sollai, F.; Masala, S.; Porcu, M.C.; Sanjust, E.; Rinaldi, A.C.; Curreli, N.; Grifi, D.; Rinaldi, A.
    Purification and characterization of an NAD(P)H:quinone oxidoreductase from Glycine max seedlings (1995), Prep. Biochem., 25, 57-67.
    View publication on PubMed
Search for more literature for 1.6.5.2

Inhibitors

Inhibitors Comment Organism Structure
3,3'-methylene-bis(4-hydroxycoumarin) 0.04 mM, complete inhibition Glycine max
Alizarin Red S 0.04 mM, 38% inhibition Glycine max
Cibacron Blue F3G-A 0.04 mM, 75% inhibition Glycine max
Procion blue MX-R 0.04 mM, 75% inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 NADPH
-
 Glycine max
0.341
-
 NADH
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21900
-
 2 * 21900, SDS-PAGE Glycine max
61600
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Agarose cibacron blue, AH-Sepharose, CM-cellulose, Sephacryl S-200 HR Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2469
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,8-dihydroxynaphthoquinone + NAD(P)H
-
 Glycine max 5,8-dihydroxynaphthohydroquinone + NAD(P)+
-
 ?
6-hydroxydopaminequinone + NAD(P)H
-
 Glycine max 6-hydroxydopaminehydroquinone + NAD(P)+
-
 ?
6-hydroxydopaquinone + NAD(P)H
-
 Glycine max 6-hydroxydopahydroquinone + NAD(P)+
-
 ?
juglone + NAD(P)H
-
 Glycine max dihydrojuglone + NAD(P)+
-
 ?
K3[Fe(CN)6] + NAD(P)H
-
 Glycine max K4[Fe(CN)6] + NAD(P)+
-
 ?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Glycine max NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADH + H+ + 2,6-dichlorophenolindophenol
-
 Glycine max NAD+ + reduced 2,6-dichlorophenolindophenol
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 21900, SDS-PAGE Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 43 decrease above Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 60% activity at pH 9.0 Glycine max