Application | Comment | Organism |
---|---|---|
synthesis | the enzyme may prove to be useful for NAD+ regeneration in the production rare L--sugars such as L-ribose, L-ribulose, and L-xylulose | Streptococcus pyogenes |
Cloned (Comment) | Organism |
---|---|
cloned into the T7 promoter-based plasmid pET28a to give pET28a-SpNox and then heterologously overexpressed in Escherichia coli BL21 (DE3) | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
C44A | 80% of wild-type activity, production of H2O2 rather than H2O | Streptococcus pyogenes |
D292A | mutant enzyme nearly loses all activity, also loses yellow color, indicating that the amino acid is important for activity | Streptococcus pyogenes |
G13A | mutant enzyme nearly loses all activity, also loses yellow color, indicating that the amino acid is important for activity | Streptococcus pyogenes |
G169A | loses nearly 100% of its activity, indicating that G169 is important for NADH binding | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Streptococcus pyogenes | |
iodoacetate | - |
Streptococcus pyogenes | |
p-chloromercuribenzoate | - |
Streptococcus pyogenes | |
Quinine | - |
Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
NADH | pH and temperature not specified in the publication | Streptococcus pyogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require a metal cofactor for activity | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Streptococcus pyogenes |
100000 | - |
gel filtration | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 NADH + H+ + O2 | Streptococcus pyogenes | - |
NAD+ + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 NADH + H+ + O2 | - |
Streptococcus pyogenes | NAD+ + 2 H2O | - |
? | |
2 NADH + H+ + O2 | no production of H2O2 | Streptococcus pyogenes | NAD+ + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
H2O-forming NADH oxidase | - |
Streptococcus pyogenes |
SpNox | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Streptococcus pyogenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 75% of maximal activity, 70°C: about 80% of maximal activity | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus pyogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | higher than 70% of the maximum activity at pH values of 6.58.5 | Streptococcus pyogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 0.9 mol of FAD per mol of subunit | Streptococcus pyogenes | |
NADH | - |
Streptococcus pyogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11000 | - |
NADH | pH and temperature not specified in the publication | Streptococcus pyogenes |