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Literature summary for 1.6.3.3 extracted from
- The NADH oxidase from the thermoacidophilic archaea Acidianus ambivalens: isolation and physicochemical characterisation (1998), Biochem. Biophys. Res. Commun., 243, 412-415.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 5 mM, slight stimulation | Acidianus ambivalens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 12 mM, slight stimulation | Acidianus ambivalens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
1 * 70000, SDS-PAGE | Acidianus ambivalens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + O2 | Acidianus ambivalens | - |
NAD+ + H2O2 | - |
? | |
| NADH + H+ + O2 | Acidianus ambivalens DSM 3772 | - |
NAD+ + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidianus ambivalens | - |
- |
- |
| Acidianus ambivalens DSM 3772 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidianus ambivalens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + O2 | - |
Acidianus ambivalens | NAD+ + H2O2 | - |
? | |
| NADH + H+ + O2 | the enzyme also acts as an NADH:ferredoxin oxidoreductase | Acidianus ambivalens | NAD+ + H2O2 | - |
? | |
| NADH + H+ + O2 | - |
Acidianus ambivalens DSM 3772 | NAD+ + H2O2 | - |
? | |
| NADH + H+ + O2 | the enzyme also acts as an NADH:ferredoxin oxidoreductase | Acidianus ambivalens DSM 3772 | NAD+ + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Acidianus ambivalens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH:O2 oxidoreductase | - |
Acidianus ambivalens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 58 | the activity increases with the temperature, as tested from 30°C to 58°C | Acidianus ambivalens |
| 58 | - |
assay at | Acidianus ambivalens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | the activity decreases with pH in the range pH 4.5 to 9.8 | Acidianus ambivalens |
| 6.5 | - |
assay at | Acidianus ambivalens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.8 | the activity decreases with pH in the range pH 4.5 to 9.8 | Acidianus ambivalens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the enzyme is FAD dependent. The activity is 5fold stimulated if the reaction assay contains FAD (0.05 mM). At FAD concentrations above 50 mM the enzyme activity is essentially exogenous flavin independent | Acidianus ambivalens | |
| NADH | NADPH can not act as the electron donor | Acidianus ambivalens | |
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