Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is applicable as a biocatalyst for NAD+ recycling | Pyrococcus horikoshii |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
beta-NADH | pH and temperature not specified in the publication | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
4 * 22000, SDS-PAGE | Pyrococcus horikoshii |
84000 | - |
gel filtration | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-NADH + H+ + O2 | Pyrococcus horikoshii | - |
beta-NAD+ + H2O2 | - |
? | |
beta-NADH + H+ + O2 | Pyrococcus horikoshii OT-3 | - |
beta-NAD+ + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58049 | - |
- |
Pyrococcus horikoshii OT-3 | O58049 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
pH and temperature not specified in the publication | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-NADH + H+ + O2 | - |
Pyrococcus horikoshii | beta-NAD+ + H2O2 | - |
? | |
beta-NADH + H+ + O2 | the enzyme is specific for beta-NADH. No activity with alpha-NADH, alpha-NADPH, or beta-NADPH | Pyrococcus horikoshii | beta-NAD+ + H2O2 | - |
? | |
beta-NADH + H+ + O2 | - |
Pyrococcus horikoshii OT-3 | beta-NAD+ + H2O2 | - |
? | |
beta-NADH + H+ + O2 | the enzyme is specific for beta-NADH. No activity with alpha-NADH, alpha-NADPH, or beta-NADPH | Pyrococcus horikoshii OT-3 | beta-NAD+ + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 22000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
PH0311 | - |
Pyrococcus horikoshii |
PhNOX | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 100 | 55°C: about 60% of maximal activity, 100°C: about 80% of maximal activity | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 5 h | Pyrococcus horikoshii |
95 | - |
half-life: 4 h | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
115 | - |
beta-NADH | pH and temperature not specified in the publication | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD-dependent enzyme | Pyrococcus horikoshii |