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Literature summary for 1.6.3.3 extracted from

  • Koh, J.-U.; Chung, H.-J.; Chang, W.-Y.; Tanokura, M.; Kong, K.-H.
    Discovery and characterization of a thermostable NADH oxidase from Pyrococcus horikoshii OT3 (2009), Bull. Korean Chem. Soc., 30, 2984-2988.
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme is applicable as a biocatalyst for NAD+ recycling Pyrococcus horikoshii

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
beta-NADH pH and temperature not specified in the publication Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
4 * 22000, SDS-PAGE Pyrococcus horikoshii
84000
-
gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-NADH + H+ + O2 Pyrococcus horikoshii
-
beta-NAD+ + H2O2
-
?
beta-NADH + H+ + O2 Pyrococcus horikoshii OT-3
-
beta-NAD+ + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58049
-
-
Pyrococcus horikoshii OT-3 O58049
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
pH and temperature not specified in the publication Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-NADH + H+ + O2
-
Pyrococcus horikoshii beta-NAD+ + H2O2
-
?
beta-NADH + H+ + O2 the enzyme is specific for beta-NADH. No activity with alpha-NADH, alpha-NADPH, or beta-NADPH Pyrococcus horikoshii beta-NAD+ + H2O2
-
?
beta-NADH + H+ + O2
-
Pyrococcus horikoshii OT-3 beta-NAD+ + H2O2
-
?
beta-NADH + H+ + O2 the enzyme is specific for beta-NADH. No activity with alpha-NADH, alpha-NADPH, or beta-NADPH Pyrococcus horikoshii OT-3 beta-NAD+ + H2O2
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 22000, SDS-PAGE Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PH0311
-
Pyrococcus horikoshii
PhNOX
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 100 55°C: about 60% of maximal activity, 100°C: about 80% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 5 h Pyrococcus horikoshii
95
-
half-life: 4 h Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
115
-
beta-NADH pH and temperature not specified in the publication Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
FAD FAD-dependent enzyme Pyrococcus horikoshii