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Literature summary for 1.6.3.1 extracted from

  • Olekhnovich, I.N.; Goodwin, A.; Hoffman, P.S.
    Characterization of the NAD(P)H oxidase and metronidazole reductase activities of the RdxA nitroreductase of Helicobacter pylori (2009), FEBS J., 276, 3354-3364.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21CodonPlus(DE3)-RIL cells Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
additional information metronidazole has no measurable inhibitory effect on the NADPH oxidase activity of RdxA Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Helicobacter pylori G27 and SS1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + O2
-
 Helicobacter pylori NADP+ + H2O2
-
 ?
NADPH + H+ + O2
-
 Helicobacter pylori G27 and SS1 NADP+ + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
RdxA the RdxA nitroreductase possesses NAD(P)H oxidase and metronidazole reductase activities Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 NADPH at 25°C, pH not specified in the publication Helicobacter pylori

General Information

General Information Comment Organism
physiological function NAD(P)H oxidase activity promotes cellular hypoxia (nonspecific reduction of metronidazole) Helicobacter pylori