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Literature summary for 1.6.2.4 extracted from

  • Im, S.C.; Waskell, L.
    The interaction of microsomal cytochrome P450 2B4 with its redox partners, cytochrome P450 reductase and cytochrome b5 (2011), Arch. Biochem. Biophys., 507, 144-153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information at low molar ratios of cytochrome b5 to cytochrome P450 reductase, the more rapid catalysis results in enhanced substrate metabolism. In contrast, at high molar ratios of cytochome b5 to cytochrome P450 reductase, cytochrome b5 inhibits activity by binding to the proximal surface of cytochrome P450 and preventing the reductase from reducing ferric cytochrome P450 to the ferrous protein, thereby aborting the catalytic reaction cycle. Cytochrome b5 stimulates catalysis by causing a conformational change in the active site, which allows the active oxidizing oxyferryl species of cytochrome P450 to be formed more rapidly than in the presence of reductase Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information deletion of 11 amino acids of the membrane anchor yields a cytosolic protein that does not bind to the endoplasmic reticulum, whereas lengthening the membrane anchor by 5 amino acids causes the protein to be transported to the plasma membrane of COS cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information at low molar ratios of cytochrome b5 to cytochrome P450 reductase, the more rapid catalysis results in enhanced substrate metabolism. In contrast, at high molar ratios of cytochome b5 to cytochrome P450 reductase, cytochrome b5 inhibits activity by binding to the proximal surface of cytochrome P450 and preventing the reductase from reducing ferric cytochrome P450 to the ferrous protein, thereby aborting the catalytic reaction cycle Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cytochrome b5 is a membrane-bound protein that is inserted into the membrane via an alpha-helix at its carboxy terminus. A random-coil linker of 15 amino acids connects the acidic soluble cytosolic heme domain to the membrane anchor Homo sapiens 16020
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microsome
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Homo sapiens
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens binding of cytochrome P450 2B4 to cytochrome b5 and cytochrome P450 reductase, binding site on cytochrome P450 2B4 for its redox partners, complex formation modelling, overview ?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
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Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information binding of cytochrome P450 2B4 to cytochrome b5 and cytochrome P450 reductase, binding site on cytochrome P450 2B4 for its redox partners, complex formation modelling, overview Homo sapiens ?
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?

Synonyms

Synonyms Comment Organism
cytochrome P450 reductase
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Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens
FMN
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Homo sapiens
additional information cytochrome P450 reductase is a membrane-bound diflavin protein, which transfers two electrons sequentially from NADPH through FAD to the FMN cofactor, which is the ultimate donor of electrons to cytochrome P450 and other acceptor proteins Homo sapiens
NADPH
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Homo sapiens

General Information

General Information Comment Organism
additional information cytochrome P450 reductase is a membrane-bound diflavin protein, which transfers two electrons sequentially from NADPH through FAD to the FMN cofactor, which is the ultimate donor of electrons to cytochrome P450 and other acceptor proteins Homo sapiens