Cloned (Comment) | Organism |
---|---|
expression of missense variant proteins lacking 27 N-terminal residues in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A462T | vmax/Km for ferricytochrome c is 85% of the wild-type value. Vmax/Km for NADPH is 69% of the wild-type value | Homo sapiens |
A485T | vmax/Km for ferricytochrome c is 36% of the wild-type value. Vmax/Km for NADPH is 51% of the wild-type value | Homo sapiens |
A503V | vmax/Km for ferricytochrome c is 67% of the wild-type value. Vmax/Km for NADPH is 56% of the wild-type value | Homo sapiens |
D211L | vmax/Km for ferricytochrome c is 27% of the wild-type value. Vmax/Km for NADPH is 59% of the wild-type value | Homo sapiens |
delE53 | vmax/Km for ferricytochrome c is 57% of the wild-type value. Vmax/Km for NADPH is 93% of the wild-type value | Homo sapiens |
E300K | vmax/Km for ferricytochrome c is 93% of the wild-type value. Vmax/Km for NADPH is 104% of the wild-type value | Homo sapiens |
G213E | vmax/Km for ferricytochrome c is 111% of the wild-type value. Vmax/Km for NADPH is 105% of the wild-type value | Homo sapiens |
P284L | vmax/Km for ferricytochrome c is 104% of the wild-type value. Vmax/Km for NADPH is 153% of the wild-type value | Homo sapiens |
P284T | vmax/Km for ferricytochrome c is 16% of the wild-type value. Vmax/Km for NADPH is 32% of the wild-type value | Homo sapiens |
P452L | vmax/Km for ferricytochrome c is 16% of the wild-type value. Vmax/Km for NADPH is 12% of the wild-type value | Homo sapiens |
P55L | vmax/Km for ferricytochrome c is 67% of the wild-type value. Vmax/Km for NADPH is 123% of the wild-type value | Homo sapiens |
R406H | vmax/Km for ferricytochrome c is 62% of the wild-type value. Vmax/Km for NADPH is 78% of the wild-type value | Homo sapiens |
R600W | vmax/Km for ferricytochrome c is 18% of the wild-type value. Vmax/Km for NADPH is 7% of the wild-type value | Homo sapiens |
V472M | vmax/Km for ferricytochrome c is 23% of the wild-type value. Vmax/Km for NADPH is 24% of the wild-type value | Homo sapiens |
Y607C | Vmax/Km for NADPH is 20% of the wild-type value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADPH | mutant enzyme Y607C | Homo sapiens | |
0.066 | - |
NADPH | mutant enzyme P284T | Homo sapiens | |
0.084 | - |
NADPH | mutant enzyme R600W | Homo sapiens | |
0.125 | - |
NADPH | mutant enzyme P452L | Homo sapiens | |
0.144 | - |
NADPH | wild-type enzyme | Homo sapiens | |
0.144 | - |
NADPH | mutant enzyme A485T | Homo sapiens | |
0.146 | - |
NADPH | mutant enzyme P55L | Homo sapiens | |
0.151 | - |
NADPH | mutant enzyme delE53 | Homo sapiens | |
0.153 | - |
NADPH | mutant enzyme D211L | Homo sapiens | |
0.155 | - |
NADPH | mutant enzyme A503V | Homo sapiens | |
0.157 | - |
NADPH | mutant enzyme P284L | Homo sapiens | |
0.169 | - |
NADPH | mutant enzyme V472M | Homo sapiens | |
0.177 | - |
NADPH | mutant enzyme R406H | Homo sapiens | |
0.196 | - |
NADPH | mutant enzyme G213E | Homo sapiens | |
0.222 | - |
NADPH | mutant enzyme E300K | Homo sapiens | |
0.276 | - |
NADPH | mutant enzyme A462T | Homo sapiens | |
0.39 | - |
ferricytochrome c | mutant enzyme R600W | Homo sapiens | |
0.92 | - |
ferricytochrome c | mutant enzyme P452L | Homo sapiens | |
0.99 | - |
ferricytochrome c | mutant enzyme A503V | Homo sapiens | |
1.15 | - |
ferricytochrome c | mutant enzyme P284T | Homo sapiens | |
1.19 | - |
ferricytochrome c | wild-type enzyme | Homo sapiens | |
1.37 | - |
ferricytochrome c | mutant enzyme V472M | Homo sapiens | |
1.61 | - |
ferricytochrome c | mutant enzyme G213E | Homo sapiens | |
1.84 | - |
ferricytochrome c | mutant enzyme A485T | Homo sapiens | |
1.86 | - |
ferricytochrome c | mutant enzyme E300K | Homo sapiens | |
2.07 | - |
ferricytochrome c | mutant enzyme R406H | Homo sapiens | |
2.09 | - |
ferricytochrome c | mutant enzyme A462T | Homo sapiens | |
2.16 | - |
ferricytochrome c | mutant enzyme delE53 | Homo sapiens | |
2.25 | - |
ferricytochrome c | mutant enzyme P284L | Homo sapiens | |
2.31 | - |
ferricytochrome c | mutant enzyme P55L | Homo sapiens | |
2.5 | 5 | ferricytochrome c | mutant enzyme D211L | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | - |
Homo sapiens | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P450 oxidoreductase | - |
Homo sapiens |
POR | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |