General Stability | Organism |
---|---|
20% glycerol stabilizes enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
ferricytochrome c | pH 7.7 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
membrane | - |
Ovis aries | 16020 | - |
microsome | - |
Bos taurus | - |
- |
microsome | - |
Ovis aries | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome P450 + NADPH | Bos taurus | component of the cytochrome P450 dependent monooxygenase system | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
2 ferricytochrome P450 + NADPH | Ovis aries | component of the cytochrome P450 dependent monooxygenase system | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Ovis aries | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
- |
Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
lung | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.5 | - |
purified enzyme with cytochrome c as substrate, pH 7.7 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | - |
Bos taurus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | - |
Ovis aries | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome P450 + NADPH | component of the cytochrome P450 dependent monooxygenase system | Bos taurus | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
2 ferricytochrome P450 + NADPH | component of the cytochrome P450 dependent monooxygenase system | Ovis aries | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
benzphetamine | demethylation in the presence of added lipid | Bos taurus | ? | - |
? | |
benzphetamine | demethylation in the presence of added lipid | Ovis aries | ? | - |
? | |
cocaine | demethylation in the presence of added lipid | Bos taurus | ? | - |
? | |
cocaine | demethylation in the presence of added lipid | Ovis aries | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-cytochrome P450 reductase | - |
Bos taurus |
NADPH-cytochrome P450 reductase | - |
Ovis aries |
NADPH-ferrihemoprotein reductase | - |
Bos taurus |
NADPH-ferrihemoprotein reductase | - |
Ovis aries |
NADPH:ferrihemoprotein oxidoreductase | - |
Bos taurus |
NADPH:ferrihemoprotein oxidoreductase | - |
Ovis aries |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
85% loss of activity within 1 h without added glycerol, 54% loss of activity within 1 h in the presence of 20% glycerol | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.8 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | together with FMN | Bos taurus | |
FAD | together with FMN | Ovis aries | |
FMN | together with FAD | Bos taurus | |
FMN | together with FAD | Ovis aries | |
NADPH | - |
Bos taurus | |
NADPH | - |
Ovis aries |