Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | cells grown on alkane or glycerol, presence of NADPH provides protection against inhibition | Candida tropicalis | |
additional information | increasing ionic strength, grown on alkane or glycerol | Candida tropicalis | |
NADP+ | - |
Candida tropicalis | |
p-chloromercuribenzoate | cells grown on alkane or glycerol, presence of NADPH provides protection against inhibition | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
ferricytochrome c | grown on glycerol | Candida tropicalis | |
0.00455 | - |
ferricytochrome c | grown on alkanes | Candida tropicalis | |
0.029 | - |
NADPH | grown on alkanes | Candida tropicalis | |
0.033 | - |
NADPH | grown on glycerol | Candida tropicalis | |
0.055 | - |
oxidized 2,6-dichlorophenolindophenol | grown on glycerol | Candida tropicalis | |
0.077 | - |
oxidized 2,6-dichlorophenolindophenol | grown on alkanes | Candida tropicalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | 68000 | gel filtration, sedimentation equilibrium centrifugation | Candida tropicalis |
67000 | - |
1 * 67000, SDS-PAGE, trypsin- or lipase-solubilized | Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Candida tropicalis | monooxygenase system only present in cells grown on alkanes | ? | - |
? | |
additional information | Candida tropicalis | monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids | ? | - |
? | |
NADPH + ferricytochrome P450 | Candida tropicalis | during hydroxylation of alkanes | ferrocytochrome P450 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
grown on alkanes or glycerol | - |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose and Sephadex G-100 column chromatography | Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | 0.3 | grown on glycerol, microsomal fraction | Candida tropicalis |
0.75 | 1.1 | grown on alkane, microsomal fraction | Candida tropicalis |
12.5 | - |
grown on alkane, electron acceptor: neotetrazolium chloride, purified enzyme | Candida tropicalis |
18.5 | - |
grown on alkane, electron acceptor: ferricyanide, purified enzyme | Candida tropicalis |
29 | - |
grown on alkane, electron acceptor: menadione, purified enzyme | Candida tropicalis |
50 | - |
grown on alkane, electron acceptor: dichlorophenolindophenol, purified enzyme | Candida tropicalis |
70 | - |
grown on alkane, electron acceptor: cytochrome c, purified enzyme | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Candida tropicalis | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: neotetrazolium chloride | Candida tropicalis | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Candida tropicalis | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Candida tropicalis | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
ferricytochrome c + NADPH + H+ | - |
Candida tropicalis | ferrocytochrome c + NADP+ | - |
r | |
additional information | monooxygenase system only present in cells grown on alkanes | Candida tropicalis | ? | - |
? | |
additional information | monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids | Candida tropicalis | ? | - |
? | |
NADPH + ferricytochrome P450 | during hydroxylation of alkanes | Candida tropicalis | ferrocytochrome P450 + NADP+ | - |
? | |
NADPH + H+ oxidized 2,6-dichlorophenolindophenol | - |
Candida tropicalis | ferrocytochrome c + reduced 2,6-dichlorophenolindophenol | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, SDS-PAGE, trypsin- or lipase-solubilized | Candida tropicalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102 | - |
ferricytochrome c | grown on alkanes | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | grown on alkane or glycerol | Candida tropicalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | less than 50% of maximal activity above and below, grown on alkane or glycerol | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | ratio FAD : FMN 1 : 1 | Candida tropicalis | |
FMN | ratio FAD : FMN 1 : 1 | Candida tropicalis | |
NADPH | - |
Candida tropicalis |