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Literature summary for 1.6.2.4 extracted from

  • Shephard, E.A.; Pike, S.F.; Rabin, B.R.; Phillips, I.R.
    A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes (1983), Anal. Biochem., 129, 430-433.
    View publication on PubMed
Search for more literature for 1.6.2.4

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus detoxification of drugs, inactivation of procarcinogens ?
-
 ?
additional information Rattus norvegicus monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids ?
-
 ?
additional information Rattus norvegicus Sprague-Dawley detoxification of drugs, inactivation of procarcinogens ?
-
 ?
additional information Rattus norvegicus Sprague-Dawley monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley strain
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley strain
-

Purification (Commentary)

Purification (Comment) Organism
single-step procedure involving affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate or two-column procedure involving chromatography on n-octylamino-Sepharose 4B followed by chromatography on 2',5'-ADP Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver phenobarbital-treated animals Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.5
-
 purification by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate Rattus norvegicus
63.8
-
 purification by n-octylamino-Sepharose 4B and 2',5'-ADP column chromatography Rattus norvegicus

Storage Stability

Storage Stability Organism
-78°C, 30 mM potassium phosphate buffer, pH 7.7, 0.1 mM EDTA, 20% glycerol, 0.4 mM PMSF Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information detoxification of drugs, inactivation of procarcinogens Rattus norvegicus ?
-
 ?
additional information monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids Rattus norvegicus ?
-
 ?
additional information detoxification of drugs, inactivation of procarcinogens Rattus norvegicus Sprague-Dawley ?
-
 ?
additional information monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids Rattus norvegicus Sprague-Dawley ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75000-76000, SDS-PAGE Rattus norvegicus