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Literature summary for 1.6.2.4 extracted from
- Purification and characterization of a membrane-bound NADPH-cytochrome c reductase capable of catalyzing menadione-dependent O2- formation in guinea pig polymorphonuclear leukocytes (1984), J. Biochem., 96, 671-678.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | formation of superoxide anion, in the presence of menadione | Cavia porcellus |  |
| EDTA | reduction of cytochrome c and formation of superoxide anion | Cavia porcellus | |
| N-ethylmaleimide | reduction of cytochrome c and formation of superoxide anion | Cavia porcellus | |
| quercetin | reduction of cytochrome c and formation of superoxide anion | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | O2-generation | Cavia porcellus | |
| 0.0053 | - |
menadione | - |
Cavia porcellus | |
| 0.025 | - |
NADPH | - |
Cavia porcellus | |
| 0.23 | - |
NADH | - |
Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Cavia porcellus | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilization with 0.2% Triton X-100, gel filtration on Sephacryl S-300 and affinity chromatography on 2',5'-ADP agarose | Cavia porcellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.68 | - |
- |
Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | additional electron acceptor: nitroblue tetrazolium salt | Cavia porcellus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Cavia porcellus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Cavia porcellus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| NADH + H+ + ferricytochrome c | - |
Cavia porcellus | NAD+ + ferrocytochrome c | - |
r | |
| NADPH + H+ + menadione | - |
Cavia porcellus | NADP+ + menadiol | - |
r | |
| NADPH + O2 | slow reaction, presence of menadione, or duroquinone, or vitamin K3 essential | Cavia porcellus | NADP+ + O2- | replacement of NADPH by NADH lowers the O2- generation | ? | |
| NADPH + O2 | slow reaction, presence of menadione, or duroquinone, or vitamin K3 essential | Cavia porcellus | NADP+ + O2- | superoxide anion | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
10 min, inhibition of O2-formation | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | O2- generation | Cavia porcellus |
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