Application | Comment | Organism |
---|---|---|
drug development | flavonoids, regarding b5 reductase inhibition, indicate a potential for significant flavonoiddrug and/or flavonoidxenobiotic interactions which may have important therapeutic and toxicological outcomes for certain drugs and/or xenobiotics. | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-catechin | - |
Bos taurus | |
(-)-epicatechin | - |
Bos taurus | |
apigenin | - |
Bos taurus | |
luteolin | - |
Bos taurus | |
luteolin-7-O-glucoside | - |
Bos taurus | |
additional information | inhibitory potencies of flavonoids on the enzyme, structure-activity relationship, overview. No inhibition by naringenin, naringin, and chrysin. Flavonoids containing two hydroxyl groups in ring B and a carbonyl group at C-4 in combination with a double bond between C-2 and C-3 produced a much stronger inhibition, whereas substitution of a hydroxyl group at C-3 is associated with a less inhibitory effect | Bos taurus | |
morin | - |
Bos taurus | |
myricetin | - |
Bos taurus | |
quercetin | - |
Bos taurus | |
quercitrin | - |
Bos taurus | |
rutin | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from liver microsomes by anion exchange chromatography and affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Bos taurus | 2 ferrocyanide + NAD+ + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome b5 reductase | - |
Bos taurus |
NADH-cytochrome b5 reductase | - |
Bos taurus |
NADH-ferricyanide reductase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Bos taurus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00011 | - |
pH 7.5, 22°C | Bos taurus | luteolin | |
0.00081 | - |
pH 7.5, 22°C | Bos taurus | morin | |
0.0011 | - |
pH 7.5, 22°C | Bos taurus | quercetin | |
0.0012 | - |
pH 7.5, 22°C | Bos taurus | quercitrin | |
0.0015 | - |
pH 7.5, 22°C | Bos taurus | myricetin | |
0.0024 | - |
pH 7.5, 22°C | Bos taurus | luteolin-7-O-glucoside | |
0.0032 | - |
pH 7.5, 22°C | Bos taurus | (-)-epicatechin | |
0.0045 | - |
pH 7.5, 22°C | Bos taurus | (+)-catechin | |
0.036 | - |
pH 7.5, 22°C | Bos taurus | apigenin | |
0.057 | - |
pH 7.5, 22°C | Bos taurus | rutin |
General Information | Comment | Organism |
---|---|---|
additional information | structure-activity relationship, overview | Bos taurus |