Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.2.2 extracted from

  • Roma, G.W.; Crowley, L.J.; Davis, C.A.; Barber, M.J.
    Mutagenesis of glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase (2005), Biochemistry, 44, 13467-13476.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
G179A mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus
G179P mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus
G179T mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus
G179V mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
ferrocytochrome b5 mutant G179P, pH 7.0 Rattus norvegicus
0.006
-
NADH wild-type, pH 7.0 Rattus norvegicus
0.007
-
ferricyanide mutant G179P, pH 7.0 Rattus norvegicus
0.007
-
ferricyanide mutant G179V, pH 7.0 Rattus norvegicus
0.008
-
ferricyanide wild-type, pH 7.0 Rattus norvegicus
0.008
-
ferricyanide mutant G179A, pH 7.0 Rattus norvegicus
0.008
-
ferrocytochrome b5 mutant G179A, pH 7.0 Rattus norvegicus
0.008
-
ferricyanide mutant G179T, pH 7.0 Rattus norvegicus
0.013
-
ferrocytochrome b5 wild-type, pH 7.0 Rattus norvegicus
0.025
-
NADH mutant G179A, pH 7.0 Rattus norvegicus
0.043
-
ferrocytochrome b5 mutant G179T, pH 7.0 Rattus norvegicus
0.107
-
ferrocytochrome b5 mutant G179V, pH 7.0 Rattus norvegicus
0.375
-
NADPH mutant G179V, pH 7.0 Rattus norvegicus
0.507
-
NADPH mutant G179T, pH 7.0 Rattus norvegicus
0.595
-
NADH mutant G179P, pH 7.0 Rattus norvegicus
0.662
-
NADH mutant G179T, pH 7.0 Rattus norvegicus
0.924
-
NADPH wild-type, pH 7.0 Rattus norvegicus
1.077
-
NADH mutant G179V, pH 7.0 Rattus norvegicus
1.36
-
NADPH mutant G179A, pH 7.0 Rattus norvegicus
2.317
-
NADPH mutant G179P, pH 7.0 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P20070
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
Rattus norvegicus 2 ferrocyanide + NAD+ + H+
-
?
2 ferricyanide + NADPH
-
Rattus norvegicus 2 ferrocyanide + NADP+ + H+
-
?
2 ferricytochrome b5 + NADH
-
Rattus norvegicus 2 ferrocytochrome b5 + NAD+ + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
NADPH mutant G179V, pH 7.0 Rattus norvegicus
10
-
ferrocytochrome b5 mutant G179P, pH 7.0 Rattus norvegicus
12
-
NADPH mutant G179T, pH 7.0 Rattus norvegicus
12
-
ferricyanide mutant G179V, pH 7.0 Rattus norvegicus
17
-
NADPH mutant G179P, pH 7.0 Rattus norvegicus
17
-
ferrocytochrome b5 mutant G179V, pH 7.0 Rattus norvegicus
18
-
ferrocytochrome b5 mutant G179T, pH 7.0 Rattus norvegicus
33
-
NADPH wild-type, pH 7.0 Rattus norvegicus
33
-
ferricyanide mutant G179T, pH 7.0 Rattus norvegicus
42
-
ferricyanide mutant G179P, pH 7.0 Rattus norvegicus
48
-
NADPH mutant G179A, pH 7.0 Rattus norvegicus
245
-
ferrocytochrome b5 mutant G179A, pH 7.0 Rattus norvegicus
400
-
ferrocytochrome b5 wild-type, pH 7.0 Rattus norvegicus
559
-
ferricyanide mutant G179A, pH 7.0 Rattus norvegicus
800
-
ferricyanide wild-type, pH 7.0 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Rattus norvegicus
NADPH
-
Rattus norvegicus