Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Atebrin | 0.5 mM, complete inhibition | Oryctolagus cuniculus | |
iodoacetamide | 1 mM, complete inhibition | Oryctolagus cuniculus | |
N-ethylmaleimide | 1 mM, 90% inhibition | Oryctolagus cuniculus | |
p-chloromercuribenzoate | 0.001 mM, complete inhibition | Oryctolagus cuniculus | |
Proflavin | 0.1 mM, 74% inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
NADH | - |
Oryctolagus cuniculus | |
0.009 | - |
ferricytochrome b5 | - |
Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | rate of reduction depends on ionic strength | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
gel filtration | Oryctolagus cuniculus |
34700 | - |
erythrocytes, cytosol, gel filtration, calculation from FAD content | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5 | ordered bi bi mechanism | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | soluble form | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
497 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, 25 mM phosphate buffer, pH 7.6, 1 mM EDTA, 0.1 mM dithiothreitol, at least a few months | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | additional acceptor: hemin | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | poor donor: NADPH | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
NADH + ferricytochrome b5 | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, soluble enzyme from erythrocyte, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no distinct optimum with phosphate or Tris-HCl buffer | Oryctolagus cuniculus |
6.4 | - |
citrate-phosphate buffer | Oryctolagus cuniculus |
6.6 | - |
Tris-maleate buffer | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per mol enzyme | Oryctolagus cuniculus | |
NADH | - |
Oryctolagus cuniculus |